College of Pharmacy, IPS Academy, Rajendra Nagar, A.B. Road, Indore 452012, Madhya Pradesh, India.
Nat Prod Res. 2011 Nov;25(19):1870-5. doi: 10.1080/14786419.2010.540760. Epub 2011 Aug 24.
This study was undertaken to isolate a flavonol, kaempferol, from the fruits of Lagenaria siceraria (bottle gourd) as a sole compound and to explore the fibrinolytic potential of the methanolic extract of the fruits of L. siceraria and the isolated compound using their in vitro activity. The fibrinolytic activity in terms of percentage of plasma clot liquefaction was determined by plasma clot lysis at 37°C in 24 h. The fibrinolytic activity of both substances was compared to the well-known thrombolytic agent streptokinase (30,000 IU). The percentage of fibrinolytic activity of the extract and isolated compound were found to be 54.72 ± 0.7210 and 77.37 ± 1.3010, respectively. Streptokinase was considered as the standard fibrinolytic enzyme for comparative purposes and had 91.46 ± 0.7625% fibrinolytic activity. The conclusion drawn in our study after testing the hypothesis by experimental procedures is that in vitro fibrinolytic activity on plasma clots is an inherent property of kaempferol isolated from the fruits of L. siceraria, and its comparison with streptokinase is a new aspect for further study.
本研究旨在从葫芦科（葫芦科）果实中分离出一种类黄酮槲皮素作为单一化合物，并利用其体外活性探索葫芦科果实甲醇提取物和分离化合物的纤维蛋白溶解潜力。纤维蛋白溶解活性以血浆凝块液化的百分比来表示，通过在 37°C 下 24 小时的血浆凝块溶解来确定。两种物质的纤维蛋白溶解活性与众所周知的溶栓剂链激酶（30000IU）进行了比较。提取物和分离化合物的纤维蛋白溶解活性分别为 54.72±0.7210%和 77.37±1.3010%。链激酶被认为是用于比较目的的标准纤维蛋白溶解酶，其纤维蛋白溶解活性为 91.46±0.7625%。通过实验程序对假设进行测试后，我们在研究中得出的结论是，对血浆凝块的体外纤维蛋白溶解活性是从葫芦科果实中分离出的槲皮素所具有的固有特性，将其与链激酶进行比较是进一步研究的一个新方面。
