School of Natural Sciences, University of California Merced, Merced, CA, USA.
Curr Opin Chem Biol. 2011 Oct;15(5):710-8. doi: 10.1016/j.cbpa.2011.07.020. Epub 2011 Aug 19.
Single molecule force spectroscopy presents a deceptively simple approach to probing interaction between molecules and molecular assemblies on the nanoscale by measuring forces that it takes to pull the molecules apart. Yet, a more detailed analysis reveals a wealth of different behaviors and interesting physics. This article aims to explore basic physical concepts behind these experiments from a strictly practical point of using these data to extract meaningful information about the interactions. It also focuses on different loading regimes in these experiments, different kinetics that they cause, and different data interpretation that is required for measurements in those regimes.
单分子力谱通过测量将分子拉开所需的力，提供了一种在纳米尺度上探测分子间相互作用和分子组装的看似简单的方法。然而，更详细的分析揭示了丰富的不同行为和有趣的物理现象。本文旨在从严格的实际应用角度探讨这些实验背后的基本物理概念，使用这些数据提取关于相互作用的有意义信息。它还侧重于这些实验中的不同加载模式、它们引起的不同动力学以及这些模式下测量所需的不同数据解释。
