细胞周期蛋白 A 荧光肽传感器的理性设计。

Rational design of a cyclin A fluorescent peptide sensor.

机构信息

Departamento de Química Orgánica y Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Unidad Asociada al CSIC, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.

出版信息

Org Biomol Chem. 2011 Oct 26;9(22):7629-32. doi: 10.1039/c1ob06009k. Epub 2011 Aug 23.

DOI:10.1039/c1ob06009k

PMID:21863156

Abstract

We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.

摘要

我们报告了一种专门针对细胞周期蛋白 A 的荧光传感器的设计和开发，细胞周期蛋白 A 是一种在细胞周期调控中起关键作用的蛋白质。计算研究提供了一个分子图像，解释了观察到的发射增加，表明当 4-DMAP 荧光团插入细胞周期蛋白 A 的疏水性结合槽时，它受到保护而不与溶剂接触。