College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, People's Republic of China.
Biol Trace Elem Res. 2012 Feb;145(2):181-8. doi: 10.1007/s12011-011-9175-x. Epub 2011 Aug 24.
Selenoprotein W (SelW) is an existing form of selenium (Se). Se influences the levels of SelW in mammals. However, little is known about the pattern of SelW expression in the gastrointestinal tract tissue of bird. The present paper describes the effects of different dietary levels of Se on the SelW mRNA expression in the gastrointestinal tract tissue of chicken. The expression levels of SelW mRNA and the Se contents in the gastrointestinal tract tissues (glandular stomach, gizzard, duodenum, small intestine, and rectum) were determined on days 15, 25, 35, 45, and 55, respectively. The results showed that the Se contents and the SelW mRNA expression were significantly higher (p < 0.05) in the high-Se group, and the Se contents and SelW mRNA expression in the low-Se group were significantly lower (p < 0.05) than in the controls. The Se contents were the highest in the duodenum and the lowest in the rectum, while the SelW mRNA expression was the highest in the gizzard and the lowest in the rectum. In addition, the SelW mRNA levels in the gastrointestinal tract tissue were found to increase in a time-dependent manner with increasing feeding time. Furthermore, the expression of the SelW mRNA in the gastrointestinal tract tissues of chickens was found to correlate with the dietary Se concentrations, but not with the tissue Se contents.
硒蛋白 W（SelW）是一种现有的硒（Se）形式。Se 影响哺乳动物中 SelW 的水平。然而，关于鸟类胃肠道组织中 SelW 表达模式的了解甚少。本文描述了不同膳食 Se 水平对鸡胃肠道组织中 SelW mRNA 表达的影响。分别在第 15、25、35、45 和 55 天测定胃肠道组织（腺胃、肌胃、十二指肠、小肠和直肠）中 SelW mRNA 的表达水平和 Se 含量。结果表明，高 Se 组的 Se 含量和 SelW mRNA 表达显著升高（p<0.05），低 Se 组的 Se 含量和 SelW mRNA 表达显著低于对照组（p<0.05）。十二指肠中的 Se 含量最高，直肠中的 Se 含量最低，而肌胃中的 SelW mRNA 表达最高，直肠中的 SelW mRNA 表达最低。此外，随着饲养时间的增加，发现胃肠道组织中的 SelW mRNA 水平呈时间依赖性增加。此外，发现鸡胃肠道组织中 SelW mRNA 的表达与膳食 Se 浓度相关，但与组织 Se 含量无关。
