College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Biol Trace Elem Res. 2011 Dec;143(3):1516-23. doi: 10.1007/s12011-011-8995-z. Epub 2011 Feb 18.
As selenium in the form of "Selenoprotein W (SelW)" is essential for the maintenance of normal liver function, the expression of SelW liver depends on the level of selenium supplied with the diet. Whereas this is well known to be the case in mammals, relatively little is known about the effect of dietary Se on the expression SelW in the livers of avian species. To investigate the effects of dietary Se levels on the SelW mRNA expression in the liver of bird, 1-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0, and 5.0 mg/kg sodium selenite (Na(2)SeO(3)) for 90 days. The livers were collected and examined for Se content and mRNA levels of SelW, Selenophosphate synthetase-1, and selenocysteine-synthase (SecS). The data indicate that, within a certain range, a Se-supplemented diet can increase the expression of SelW and the mRNA levels of SecS, and also, that the transcription of SelW is very sensitive to dietary Se.
由于硒以“硒蛋白 W（SelW）”的形式存在对于维持正常肝功能至关重要，因此 SelW 肝脏的表达取决于饮食中提供的硒水平。虽然这在哺乳动物中是众所周知的，但关于饮食硒对禽类肝脏中 SelW 表达的影响知之甚少。为了研究饮食硒水平对鸟类肝脏 SelW mRNA 表达的影响，1 日龄雄性小鸡分别喂食商业饮食或含 1.0、2.0、3.0 和 5.0mg/kg 亚硒酸钠（Na2SeO3）的补充硒饮食90 天。收集肝脏并检查硒含量和 SelW、硒代磷酸合成酶 1 和硒代半胱氨酸合成酶（SecS）的 mRNA 水平。数据表明，在一定范围内，补充硒饮食可以增加 SelW 的表达和 SecS 的 mRNA 水平，并且 SelW 的转录对饮食硒非常敏感。
