College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, China.
Biol Trace Elem Res. 2013 Jul;154(1):127-33. doi: 10.1007/s12011-013-9711-y. Epub 2013 Jun 1.
We sought to elucidate the effects of different concentrations of dietary selenium on calcium ion release, MLCK levels, and muscle contraction in the uterine smooth muscle of rats. The selenium (Se) content of blood and of uterine smooth muscle tissues was detected by fluorescence spectrophotometry. Ca(2+) content was measured by atomic absorption spectroscopy. Calmodulin (CaM) and MLCK RNA and protein levels were analyzed by quantitative real-time polymerase chain reaction and Western blot, respectively. Dietary Se intake increased the Se levels in the blood and in uterine smooth muscle tissues and increased the Ca(2+) concentration in uterine smooth muscle tissues. The addition of Se also promoted CaM expression and enhanced MLCK activation in uterine smooth muscle tissues. In conclusion, Ca(2+), CaM, and MLCK were regulated by Se in uterine smooth muscle; Se plays a major role in regulating smooth muscle contraction in the uterus.
我们旨在阐明不同浓度的膳食硒对钙离子释放、肌球蛋白轻链激酶（MLCK）水平和大鼠子宫平滑肌收缩的影响。通过荧光分光光度法检测血液和子宫平滑肌组织中的硒（Se）含量。通过原子吸收光谱法测量钙离子（Ca2+）含量。通过定量实时聚合酶链反应和 Western blot 分别分析钙调蛋白（CaM）和 MLCK RNA 和蛋白质水平。膳食 Se 摄入增加了血液和子宫平滑肌组织中的 Se 水平，并增加了子宫平滑肌组织中的 Ca2+浓度。添加 Se 还促进了子宫平滑肌组织中 CaM 的表达，并增强了 MLCK 的激活。总之，Se 调节了子宫平滑肌中的 Ca2+、CaM 和 MLCK；Se 在调节子宫平滑肌收缩方面起着重要作用。
