Pinto A C, Melo-Barbosa H P, Miyoshi A, Silva A, Azevedo V
Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Genet Mol Res. 2011;10(3):1707-18. doi: 10.4238/vol10-3gmr1554.
The large number of microbial genomes deposited in databanks has opened the door for in-depth studies of organisms, including post-genomics investigations. Thanks to new generation sequencing technology, these studies have made advances that have lead to extraordinary discoveries in bacterial transcriptomics. In this review, we describe bacterial RNA sequencing studies that use these new techniques. We also examined the advantages and biases of these new generation technologies; advances in bioinformatics make it possible to overcome the biases, providing interesting and surprising results.
数据库中存储的大量微生物基因组为包括后基因组学研究在内的生物体深入研究打开了大门。得益于新一代测序技术，这些研究取得了进展，在细菌转录组学方面带来了非凡的发现。在本综述中，我们描述了使用这些新技术的细菌RNA测序研究。我们还研究了这些新一代技术的优点和偏差；生物信息学的进展使得克服这些偏差成为可能，从而提供了有趣且令人惊讶的结果。
