Institute of Food Research, Norwich, UK.
FEMS Microbiol Lett. 2010 Jan;302(1):1-7. doi: 10.1111/j.1574-6968.2009.01767.x. Epub 2009 Aug 21.
Over the past 15 years, microbial functional genomics has been made possible by the combined power of genome sequencing and microarray technology. However, we are now approaching the technical limits of microarray technology, and microarrays are now being superseded by transcriptomics based on high-throughput (next generation) DNA-sequencing technologies. The term RNA-seq has been coined to represent transcriptomics by next-generation sequencing. Although pioneered on eukaryotic organisms due to the relative ease of working with eukaryotic mRNA, the RNA-seq technology is now being ported to microbial systems. This review will discuss the opportunities of RNA-seq transcriptome sequencing for microorganisms, and also aims to identify challenges and pitfalls of the use of this new technology in microorganisms.
在过去的 15 年中，基因组测序和微阵列技术的结合使得微生物功能基因组学成为可能。然而，我们现在已经接近微阵列技术的技术极限，而微阵列技术正逐渐被基于高通量（下一代）DNA 测序技术的转录组学所取代。RNA-seq 这个术语被用来表示基于下一代测序的转录组学。尽管由于真核 mRNA 相对容易处理，该技术首先在真核生物中得到了应用，但现在该 RNA-seq 技术正在被移植到微生物系统中。本文将讨论 RNA-seq 转录组测序在微生物中的应用机会，并旨在确定该新技术在微生物中应用的挑战和陷阱。
