[乙肝病毒携带者肝活检标本中乙肝病毒共价闭合环状DNA的定量分析：与血清学标志物及血清乙肝病毒DNA的相关性]

[Quantitative analysis of HBV cccDNA from liver biopsy specimens in HBV carriers: correlation with serological makers and sera HBV DNA].

作者信息

Zhang Lin, Wu Feng, Shi Li-lan, Dou Xiao-guang

机构信息

Department of Infectious Disease, Shengjing Hospital of China Medical University, Shenyang 110004, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Apr;25(2):112-3.

PMID:21863632

Abstract

OBJECTIVE

To investigate the correlation of sera HBV DNA and serological makers with hepatic tissue HBVcccDNA in chronic HBV carriers.

METHODS

Real time fluorescence quantitative polymerase chain reaction (RT-PCR) were used to detect HBV covalently closed circular DNA (cccDNA) and total intrahepatic HBV DNA from 30 needle-biopsy specimens as well as HBV DNA in sera in chronic HBV carriers. Quantification of the HBsAg, HBeAg in sera were quantified using Chemiluminescence immunoassay.

RESULTS

HBVcccDNA can be detected in chronic HBV carriers, which rang from 3.15 x 10(3) copies/mg to 1.06 x 10(7) copies/mg. There was a positive correlation between the cccDNA and HBVtDNA (r = 0.375, P < 0.05), but there was no correlation between the cccDNA and sera HBV DNA (P = 0.174). There was a positive correlation between cccDNA and sera HBsAg quantification (r = 0.562, P < 0.001) but no correlation with sera HBeAg qantification (r = 0.152, P > 0.05).

CONCLUSION

HBV cccDNA can be replicated stably in hepatic tissue in all chronic HBV carriers. HBV DNA in sera can not be indicated hepatic tissue cccDNA level. While HBsAg quantification in sera can be used as a marker of cccDNA quantification in hepatic tissue to some extent.

摘要

目的

探讨慢性HBV携带者血清HBV DNA及血清学标志物与肝组织HBVcccDNA的相关性。

方法

采用实时荧光定量聚合酶链反应（RT-PCR）检测30例慢性HBV携带者肝穿刺活检标本中的HBV共价闭合环状DNA（cccDNA）、肝内总HBV DNA以及血清中的HBV DNA。采用化学发光免疫分析法对血清中的HBsAg、HBeAg进行定量分析。

结果

慢性HBV携带者肝组织中可检测到HBVcccDNA，其含量范围为3.15×10³拷贝/毫克至1.06×10⁷拷贝/毫克。cccDNA与HBVtDNA之间呈正相关（r = 0.375，P < 0.05），但cccDNA与血清HBV DNA之间无相关性（P = 0.174）。cccDNA与血清HBsAg定量之间呈正相关（r = 0.562，P < 0.001），与血清HBeAg定量无相关性（r = 0.152，P > 0.05）。