Zhao Jing, Xu Jun, Chen Wei-wei, Li Yong-li, Tang Yan, Li Jia, Wang Hai-bin, Guo Tong-sheng, Zhao Min, Li Bo-an, Mao Yuan-li
Liver Failure Treatment and Research Center, 302nd Hospital of PLA, Beijing 100039, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Apr;25(2):140-2.
Assessment of detection of IgM antibodies for human enterovirus 71 (EV 71) in early diagnosis for the hand, foot and mouth disease (HFMD).
The sera and throat swabs from 38 patients which were clinical diagnosis as HFMD, were continuous daily collected in our hospital in 2010. These specimens were detected by EV 71 IgM antibodies assay, real time RT-PCR methods for EV 71 and Enterovirus.
Among 38 HFMD patients, the cumulative positive rates of EV 71 IgM antibodies were: 60.5% on day 1, 71.1% on day 2, 81.5% in the first 3-4 days, 92.1% on day 5, 92.1% on day 6, and the positive rate of nucleic acid detected by the real time RT-PCR for EV 71 and Enterovirus were 60.5%, 73.6%.
The positive rate of EV 71 IgM antibodies in the hand, foot and mouth disease just can occur on day 1, and reach to peak on day 5, which can be used as one of indicators of early diagnosis of hand, foot and mouth disease.
评估人肠道病毒71型（EV 71）IgM抗体检测在手足口病（HFMD）早期诊断中的作用。
2010年在我院连续每日收集38例临床诊断为手足口病患者的血清和咽拭子。采用EV 71 IgM抗体检测法、EV 71和肠道病毒实时RT-PCR方法对这些标本进行检测。
38例手足口病患者中，EV 71 IgM抗体累积阳性率分别为：第1天60.5%，第2天71.1%，前3 - 4天81.5%，第5天92.1%，第6天92.1%；EV 71和肠道病毒实时RT-PCR核酸检测阳性率分别为60.5%、73.6%。
手足口病中EV 71 IgM抗体阳性率在第1天即可出现，第5天达到高峰，可作为手足口病早期诊断指标之一。
