[The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD].

OBJECTIVE

To establish the real-time fluorescent PCR method for detecting enterovirus, enterovirus 71 and Coxsackievirus A 16 nucleic acid.

METHODS

Primers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR.

RESULTS

The real-time fluorescent PCR positive rates of EV, EV71 and Cox A16 were 73.7%, 60.5%, 13.2%; the conventional RT-PCR were 71.1%, 55.3%, 13.2%. There were no significant differences between the two methods.

CONCLUSION

The real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully.