Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Drug Deliv. 2011 Nov;18(8):586-98. doi: 10.3109/10717544.2011.600783. Epub 2011 Aug 24.
The methods of protein and drug delivery for the treatment of cancer, genetic diseases and diagnostics were summarized. The potential of protein transduction is discussed and the recent developments in the field are reviewed. An overview is provided of the non-viral delivery methods such as liposomes, polymer-based delivery, cell-penetrating peptides, bacterial secretion, cells, virosomes, physical methods including electroporation, microinjection, osmotic lysis, nanoparticles, sonoporation to locally inject therapeutic molecules. The characteristic properties of non-viral vectors and their use for the delivery of therapeutic molecules for the diagnosis and treatment of disorders and to target tumors are also discussed. The potential of the transduced peptides and proteins was used as new therapeutic compounds against infectious diseases, to complement deficiencies in specific genes, to specifically kill tumour cells, for gene therapy. The protein delivery vectors can enhance the transfection at low concentrations and help to develop future gene delivery systems with reduced toxicity. Vitamin B12, folic acid, biotin, and riboflavin are essential in the treatment of cancer. Ultrasound has a potential in the delivery of therapeutic agents. The new developing technologies of drug delivery and targeting offer the possibility to improve the therapeutic possibilities of the existing drugs and to develop novel therapeutics.
本文总结了用于治疗癌症、遗传疾病和诊断的蛋白质和药物传递方法。讨论了蛋白质转导的潜力，并回顾了该领域的最新发展。概述了非病毒传递方法，如脂质体、基于聚合物的传递、穿透肽、细菌分泌、细胞、病毒体、物理方法包括电穿孔、微注射、渗透压裂解、纳米颗粒、声孔法，用于局部注射治疗分子。还讨论了非病毒载体的特征性质及其用于递送至病变的治疗分子以靶向肿瘤的用途，用于诊断和治疗。转导肽和蛋白质的潜力可用于针对传染病的新型治疗化合物，以补充特定基因的缺陷，特异性杀伤肿瘤细胞，用于基因治疗。蛋白质传递载体可以在低浓度下增强转染，并有助于开发具有降低毒性的新型基因传递系统。维生素 B12、叶酸、生物素和核黄素在癌症治疗中必不可少。超声在传递治疗剂方面具有潜力。药物传递和靶向的新技术发展提供了改善现有药物治疗可能性和开发新疗法的可能性。
