Department of Biochemistry and Biotechnology, Annamalai University, Tamil Nadu, India.
Chem Biol Interact. 2011 Oct 15;194(1):40-7. doi: 10.1016/j.cbi.2011.08.004. Epub 2011 Aug 16.
Arsenic compounds are classified as toxicants and human carcinogens. Environmental exposure to arsenic imposes a big health issue worldwide. Sinapic acid is a phenylpropanoid compound and is found in various herbal materials and high-bran cereals. It has been reported that sinapic acid has antioxidant efficacy as metal chelators due to the orientation of functional groups. However, it has not yet been examined in experimental animals. In light of this fact, the purpose of this study was to characterize the protective role of sinapic acid against arsenic induced toxicity in rats. Rats were orally treated with arsenic alone (5mg/kg body weight (bw)/day) plus sinapic acid at different doses (10, 20 and 40mg/kg bw/day) for 30days. Hepatotoxicity was measured by the increased activities of serum hepatospecific enzymes namely aspartate transaminase, alanine transaminase, alkaline phosphatase, gamma glutamyl transferase, lactate dehydrogenase and total bilirubin along with increased elevation of lipid peroxidative markers, thiobarbituric acid reactive substances, lipid hydroperoxides, protein carbonyl content and conjugated dienes. The toxic effect of arsenic was also indicated by significantly decreased activities of enzymatic antioxidants like superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase and glucose-6-phosphate dehydrogenase along with non-enzymatic antioxidant like reduced glutathione. Administration of sinapic acid exhibited significant reversal of arsenic induced toxicity in hepatic tissue. The effect at a dose of 40mg/kgbw/day was more pronounced than the other two doses (10 and 20mg/kgbw/day). All these changes were supported by reduction of arsenic concentration and histopathological observations of the liver. These results suggest that sinapic acid has a protective effect over arsenic induced toxicity in rat.
砷化合物被归类为有毒物质和人类致癌物。环境暴露于砷会在全球范围内造成严重的健康问题。芥子酸是一种苯丙烷类化合物，存在于各种草药材料和高麸谷物中。据报道，芥子酸由于官能团的取向，作为金属螯合剂具有抗氧化功效。然而，它尚未在实验动物中进行过检验。鉴于此事实，本研究的目的是表征芥子酸对大鼠砷诱导毒性的保护作用。大鼠经口给予单独的砷（5mg/kg 体重/天）加芥子酸在不同剂量（10、20 和 40mg/kg bw/天）30 天。通过血清肝特异性酶的活性升高来衡量肝毒性，即天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、γ-谷氨酰转移酶、乳酸脱氢酶和总胆红素，以及脂质过氧化标记物、硫代巴比妥酸反应物质、脂质过氧化物、蛋白质羰基含量和共轭二烯的升高。砷的毒性作用还表现为酶抗氧化剂如超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶、谷胱甘肽还原酶和葡萄糖-6-磷酸脱氢酶的活性显著降低，以及非酶抗氧化剂如还原型谷胱甘肽的活性降低。芥子酸的给药表现出对肝组织中砷诱导毒性的显著逆转作用。剂量为 40mg/kgbw/天的效果比其他两个剂量（10 和 20mg/kgbw/天）更为明显。所有这些变化都得到了降低砷浓度和肝脏组织病理学观察的支持。这些结果表明，芥子酸对大鼠砷诱导的毒性具有保护作用。
