Favero Gabriele, Fusco Giovanni, Mazzei Franco, Tasca Federico, Antiochia Riccarda
Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Roma, Italy.
Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Roma, Italy.
Nanomaterials (Basel). 2015 Nov 20;5(4):1995-2006. doi: 10.3390/nano5041995.
The aim of this work is to show how the integration of gold nanoparticles (AuNPs) into multi-wall-carbon-nanotubes (MWCNTs) based screen-printed electrodes and into graphene-based screen-printed electrodes (GPHs) could represent a potential way to further enhance the electrochemical properties of those electrodes based on nanoparticles. Laccase from (TvL) was immobilized over MWCNTs and GPH previously modified with AuNPs (of 5 and 10 nm). The characterization of the modified electrode surface has been carried out by cyclic voltammetry. The results showed that the use of AuNPs for modification of both graphene and MWCNTs screen-printed electrode surfaces would increase the electrochemical performances of the electrodes. MWCNTs showed better results than GPH in terms of higher electroactive area formation after modification with AuNPs. The two modified nanostructured electrodes were successively proven to efficiently immobilize the TvL; the electrochemical sensing properties of the GPH- and MWCNT-based AuNPs-TvL biosensors were investigated by choosing 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic-acid diammonium salt (ABTS), catechol and caffeic acid as laccase mediators; and the kinetic parameters of the laccase biosensor were carefully evaluated.
这项工作的目的是展示将金纳米颗粒（AuNPs）整合到基于多壁碳纳米管（MWCNTs）的丝网印刷电极以及基于石墨烯的丝网印刷电极（GPHs）中，如何可能成为进一步增强那些基于纳米颗粒的电极电化学性能的潜在方法。来自嗜热栖热菌（TvL）的漆酶被固定在先前用5纳米和10纳米的AuNPs修饰的MWCNTs和GPH上。通过循环伏安法对修饰电极表面进行了表征。结果表明，使用AuNPs修饰石墨烯和MWCNTs丝网印刷电极表面都会提高电极的电化学性能。在用AuNPs修饰后，就更高的电活性面积形成而言，MWCNTs比GPH表现出更好的结果。这两种修饰的纳米结构电极被相继证明能有效地固定TvL；通过选择2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐（ABTS）、儿茶酚和咖啡酸作为漆酶介体，研究了基于GPH和MWCNT的AuNPs-TvL生物传感器的电化学传感特性；并仔细评估了漆酶生物传感器的动力学参数。
