Key Laboratory of Eco-Textiles of Ministry of Education, Jiangnan University, Wuxi 214122, P. R. China.
Beilstein J Nanotechnol. 2014 Mar 24;5:346-54. doi: 10.3762/bjnano.5.39. eCollection 2014.
Carbon nanofibers (CNFs) were prepared by combining electrospinning with a high-temperature carbonization technique. And a polyphenol biosensor was fabricated by blending the obtained CNFs with laccase and Nafion. Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscope (FE-SEM) were, respectively, employed to investigate the structures and morphologies of the CNFs and of the mixtures. Cyclic voltammetry and chronoamperometry were employed to study the electrocatalysis of the catechol biosensor. The results indicated that the sensitivity of the biosensor was 41 µA·mM(-1), the detection limit was 0.63 µM, the linear range was 1-1310 µM and the response time was within 2 seconds, which excelled most other laccase-based biosensor reported. Furthermore, the biosensor showed good repeatability, reproducibility, stability and tolerance to interferences. This novel biosensor also demonstrated its promising application in detecting catechol in real water samples.
碳纳米纤维（CNFs）是通过静电纺丝与高温碳化技术相结合制备的。将得到的 CNFs 与漆酶和 Nafion 混合，制备了一种多酚生物传感器。拉曼光谱、傅里叶变换红外光谱（FTIR）和场发射扫描电子显微镜（FE-SEM）分别用于研究 CNFs 和混合物的结构和形态。循环伏安法和计时电流法用于研究儿茶酚生物传感器的电催化性能。结果表明，该生物传感器的灵敏度为 41µA·mM(-1)，检测限为 0.63µM，线性范围为 1-1310µM，响应时间在 2 秒内，优于大多数其他报道的漆酶生物传感器。此外，该生物传感器还表现出良好的重复性、重现性、稳定性和抗干扰能力。这种新型生物传感器还展示了在检测实际水样中的儿茶酚方面的应用前景。
