Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Anal Bioanal Chem. 2012 Feb;402(5):1749-58. doi: 10.1007/s00216-011-5317-4. Epub 2011 Aug 25.
In this review, the preparation and properties of protein architectures constructed by layer-by-layer (LbL) deposition through avidin-biotin and concanavalin A (Con A)-sugar interactions are discussed in relation to their use for optical and electrochemical biosensors. LbL films can be constructed through the alternate deposition of avidin and biotin-labeled enzymes on the surfaces of optical probes and electrodes. The enzymes retain their catalytic activity, resulting in the formation of optical and electrochemical biosensors. Alternatively, Con A can be used to construct enzyme-containing LbL films and microcapsules using sugar-labeled enzymes. Some enzymes such as glucose oxidase and horseradish peroxidase can be used for this purpose without labeling with sugar, because these enzymes contain intrinsic hydrocarbon chains on their molecular surfaces. The Con A/enzyme LbL architectures were successfully used to develop biosensors sensitive to specific substrates of the enzyme. In addition, Con A-based films can be used for the optical and electrochemical detection of sugars.
在这篇综述中，讨论了通过亲和素-生物素和伴刀豆球蛋白 A（Con A）-糖相互作用逐层（LbL）沉积制备蛋白质结构的方法及其在光学和电化学生物传感器中的应用。LbL 薄膜可以通过在光学探针和电极表面上交替沉积亲和素和生物素标记的酶来构建。酶保留其催化活性，从而形成光学和电化学生物传感器。或者，可以使用糖标记的酶使用 Con A 来构建含有酶的 LbL 薄膜和微胶囊。某些酶，如葡萄糖氧化酶和辣根过氧化物酶，可以在不进行糖标记的情况下用于此目的，因为这些酶在其分子表面上含有内在的碳氢链。Con A/酶 LbL 结构成功地用于开发对酶的特定底物敏感的生物传感器。此外，基于 Con A 的薄膜可用于光学和电化学检测糖。
