Yoshida Kentaro, Sato Katsuhiko, Ono Tetsuya, Dairaku Takenori, Kashiwagi Yoshitomo
School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomita-machi, Koriyama, Fukushima 963-8611, Japan.
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Polymers (Basel). 2018 Jul 24;10(8):812. doi: 10.3390/polym10080812.
Thin films were prepared using layer-by-layer (LbL) deposition of Nafion (NAF) and polycations such as poly(allylamine hydrochloride) (PAH), poly(ethyleneimine) (PEI), and poly(diallydimethylammonium chloride) (PDDA). Insulin was then adsorbed on the NAF-polycation LbL films by immersion in an insulin solution. The NAF-polycation LbL films were characterized using a quartz crystal microbalance and an atomic force microscope. The release of insulin from the LbL films was characterized using UV-visible adsorption spectroscopy and fluorescence emission spectroscopy. The greatest amount of insulin was adsorbed on the NAF-PAH LbL film. The amount of insulin adsorbed on the (NAF/PAH)₅NAF LbL films by immersion in a 1 mg mL insulin solution at pH 7.4 was 61.8 µg cm. The amount of insulin released from the LbL films was higher when immersed in insulin solutions at pH 2.0 and pH 9.0 than at pH 7.4. Therefore, NAF-polycations could be employed as insulin delivery LbL films under mild conditions and as an insulin release control system according to pH change.
通过层层（LbL）沉积法制备了包含Nafion（NAF）和聚阳离子如聚（烯丙胺盐酸盐）（PAH）、聚（乙烯亚胺）（PEI）以及聚（二烯丙基二甲基氯化铵）（PDDA）的薄膜。然后将薄膜浸入胰岛素溶液中，使胰岛素吸附在NAF - 聚阳离子LbL薄膜上。使用石英晶体微天平以及原子力显微镜对NAF - 聚阳离子LbL薄膜进行表征。使用紫外可见吸收光谱和荧光发射光谱对LbL薄膜中胰岛素的释放情况进行表征。胰岛素在NAF - PAH LbL薄膜上的吸附量最大。在pH 7.4条件下，将（NAF/PAH）₅NAF LbL薄膜浸入1 mg/mL胰岛素溶液中时，胰岛素的吸附量为61.8 μg/cm²。当将LbL薄膜浸入pH 2.0和pH 9.0的胰岛素溶液中时，胰岛素从LbL薄膜中的释放量高于在pH 7.4时的释放量。因此，NAF - 聚阳离子可在温和条件下用作胰岛素递送LbL薄膜，并可作为根据pH变化的胰岛素释放控制系统。
