Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, Bundoora, Vic. 3083, Australia.
Anal Biochem. 2011 Nov 15;418(2):253-9. doi: 10.1016/j.ab.2011.08.004. Epub 2011 Aug 9.
Current methods for measuring fructan levels in plant tissues are time-consuming and costly. They often involve multiple or sequential extractions, enzymatic or acid hydrolysis of fructan polymers, and multiple HPLC runs to quantify fructan-derived hexoses. Here we describe a new method that requires a single extraction step, followed by selective precipitation of fructans by acetone, acid hydrolysis of the precipitate, and a short (10 min) HPLC run to complete the procedure. We used perennial ryegrass samples to show that the new method has similar sensitivity, but better reproducibility, than a more complex method that is widely used. We have used the new method to study developmentally related changes in fructan levels in glasshouse-grown perennial ryegrass plants.
目前测量植物组织中果聚糖水平的方法既耗时又昂贵。它们通常涉及多次或连续的提取、果聚糖聚合物的酶解或酸解，以及多次 HPLC 运行以定量果糖衍生的己糖。在这里，我们描述了一种新方法，它只需要进行一次提取步骤，然后通过丙酮选择性沉淀果聚糖，沉淀酸解，以及进行 10 分钟的 HPLC 运行即可完成整个过程。我们使用多年生黑麦草样本表明，与广泛使用的更复杂的方法相比，新方法具有相似的灵敏度，但更好的重现性。我们已经使用新方法研究了温室中生长的多年生黑麦草植物中与发育相关的果聚糖水平变化。
