Ma Jing, Zhang Tao, Zhuang Ping, Zhang Long Z, Liu Ting
School of Life Science, East China Normal University, Shanghai 200062, PR China.
Mar Genomics. 2011 Sep;4(3):173-9. doi: 10.1016/j.margen.2011.04.002. Epub 2011 Jul 8.
Chinese sturgeon Acipenser sinensis belongs to the family Acipenseridae, an ancient species of actinopterygian fishes. In order to advance molecular research on its reproduction, ontogenetic development, we were seeking for genomic information in the NCBI expressed sequence tag (EST database). We found 3384 indentified cDNA sequences which were assembled into 861 unigenes. Blast analysis revealed 301 unigenes shared high similarity with genes in the public databases, and these were classified into three groups: 202 known genes, 81 putative genes and 8 unknown genes. The remainder (560 genes) had no significant match to any protein sequence. Further, 255 unigenes and 333 unmatched unigenes were annotated with Gene Ontology (GO), which could be classified into cellular component, molecular function, and biological process. Among the known genes, the hormone genes pomc A (proopiomelanocortin), pomc B, GtH alpha I subunit (gonadotropin hormone), GtH alpha II subunit and GH (growth hormone) were present in this library. Comparison of the Chinese sturgeon proteins (GH, GtH alpha subunit and POMC) to proteins of other species showed higher levels of homology among sturgeon species. We performed five hormone related genes including GnRHRI (gonadotropin-releasing hormone receptor I), cpH (carboxypeptidase H), ppiB (peptidylprolyl isomerase B), stmn3 (stathmin-like 3), 7B2 (neuroendocrine protein 7B2), and four novel genes (contig 192, 177, 170 and 168) a semi-quantitative RT-PCR on different tissues from Chinese sturgeon.
中华鲟属于鲟科，是一种古老的硬骨鱼类。为了推进对其繁殖、个体发育的分子研究，我们在NCBI表达序列标签（EST数据库）中寻找基因组信息。我们发现了3384个已鉴定的cDNA序列，这些序列被组装成861个单基因。Blast分析显示，301个单基因与公共数据库中的基因具有高度相似性，这些基因被分为三组：202个已知基因、81个推定基因和8个未知基因。其余（560个基因）与任何蛋白质序列均无显著匹配。此外，255个单基因和333个未匹配的单基因用基因本体论（GO）进行了注释，可分为细胞成分、分子功能和生物学过程。在已知基因中，该文库中存在激素基因促肾上腺皮质激素原A（proopiomelanocortin）、促肾上腺皮质激素原B、促性腺激素α I亚基（gonadotropin hormone）、促性腺激素α II亚基和生长激素（growth hormone）。中华鲟蛋白质（生长激素、促性腺激素α亚基和促肾上腺皮质激素原）与其他物种蛋白质的比较显示，鲟鱼物种之间的同源性水平更高。我们对中华鲟不同组织中的五个激素相关基因，包括促性腺激素释放激素受体I（gonadotropin-releasing hormone receptor I）、羧肽酶H（carboxypeptidase H）、肽基脯氨酰异构酶B（peptidylprolyl isomerase B）、类微管相关蛋白3（stathmin-like 3）、神经内分泌蛋白7B2（neuroendocrine protein 7B2）以及四个新基因（重叠群192、177、170和168）进行了半定量RT-PCR。
