Centre de Recherche en Cancérologie de Marseille, Unité Mixle de Recherche, Marseille, France.
Blood. 2011 Oct 27;118(17):4609-19. doi: 10.1182/blood-2010-12-323972. Epub 2011 Aug 25.
In adult mammals, hematopoietic stem cells (HSCs) reside in the bone marrow (BM) and are maintained in a quiescent and undifferentiated state through adhesive interactions with specialized microenvironmental niches. Although junctional adhesion molecule-C (JAM-C) is expressed by HSCs, its function in adult hematopoiesis remains elusive. Here, we show that HSCs adhere to JAM-B expressed by BM stromal cells in a JAM-C dependent manner. The interaction regulates the interplay between HSCs and BM stromal cells as illustrated by the decreased pool of quiescent HSCs observed in jam-b deficient mice. We further show that this is probably because of alterations of BM stromal compartments and changes in SDF-1α BM content in jam-b(-/-) mice, suggesting that JAM-B is an active player in the maintenance of the BM stromal microenvironment.
在成年哺乳动物中，造血干细胞 (HSCs) 存在于骨髓 (BM) 中，并通过与专门的微环境龛的黏附相互作用，维持在静止和未分化的状态。尽管 HSCs 表达连接黏附分子-C (JAM-C)，但其在成人造血中的功能仍不清楚。在这里，我们表明 HSCs 以 JAM-C 依赖的方式与 BM 基质细胞表达的 JAM-B 黏附。这种相互作用调节了 HSCs 和 BM 基质细胞之间的相互作用，如在 jam-b 缺陷小鼠中观察到静止 HSCs 池减少所说明的那样。我们进一步表明，这可能是由于 BM 基质隔室的改变和 jam-b(-/-) 小鼠中 SDF-1α BM 含量的变化，表明 JAM-B 是维持 BM 基质微环境的活跃参与者。
