Department of Irradiation Technology, Nuclear Research Center of Algiers, BP-399, Algiers, Algeria.
J Mater Sci Mater Med. 2011 Nov;22(11):2505-12. doi: 10.1007/s10856-011-4421-5. Epub 2011 Aug 26.
Poly (vinyl alcohol) hydrogels containing different concentrations of chitosan with molecular weight of 471 and 101 kDa were crosslinked by gamma irradiation at a dose of 25 kGy. The swelling behavior, gel content and morphological structure of the blend were investigated. The antibacterial effect, as a function of chitosan content and molecular weight in the hydrogel, was investigated against Escherichia coli and Bacillus subtilis. With increasing chitosan content the equilibrium degree of swelling of the blend increased and the gel fraction decreased. Results of antibacterial activity of chitosan revealed that chitosan was more effective in inhibiting growth of gram positive bacteria than that of gram negative ones. It was observed that, the chitosan content as well as its molecular weight has a direct influence on bacteria growth inhibition. The higher the chitosan content in the blend and the higher its initial molecular weight, the larger was the inhibition zone diameter. The bacteria growth inhibition was attributed to the diffusion of entrapped chitosan from the hydrogel blend to the culture medium.
聚（聚乙烯醇）水凝胶含有不同浓度的壳聚糖，分子量分别为 471 和 101 kDa，通过 25 kGy 的γ射线交联。研究了共混物的溶胀行为、凝胶含量和形态结构。研究了壳聚糖含量和分子量对水凝胶的抗菌效果，以大肠杆菌和枯草芽孢杆菌为研究对象。随着壳聚糖含量的增加，共混物的平衡溶胀度增加，凝胶分数降低。壳聚糖的抗菌活性结果表明，壳聚糖对革兰氏阳性菌的抑制作用比对革兰氏阴性菌的抑制作用更强。观察到，壳聚糖的含量及其分子量对细菌生长抑制有直接影响。共混物中壳聚糖含量越高，初始分子量越高，抑菌圈直径越大。细菌生长抑制归因于从水凝胶共混物中扩散到培养基中的包埋壳聚糖。
