Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
Wuhan Engineering Science and Technology Institute, Wuhan, 430019, China.
Macromol Biosci. 2021 Apr;21(4):e2000398. doi: 10.1002/mabi.202000398. Epub 2021 Feb 24.
In this work, a 3D printed double-network (DN) hydrogel scaffold is designed with chitosan (CS) and polyvinyl alcohol (PVA) as the framework matrix. The addition of PVA into the CS-based hydrogel clearly enhances the mechanical properties and lowers the swelling behaviors of the hydrogels. The crosslinking of CS with genipin can perform the pre-crosslinking to improve the viscosity and 3D printability of the hydrogel precursor, while increasing the PVA content results in lowering the viscosity and 3D printability of the pre-crosslinked hydrogel. The antibacterial property results of the DN hydrogel display that the hydrogel have favorable long-lasting antibacterial ability. The appropriate pre-crosslinked hydrogel with the CS/PVA mass ratio of 3:10 and pre-crosslinking time of 7 h is used for 3D printing to prepare the 3D printed porous DN hydrogels. Moreover, the anti-tumor drug doxorubicin (DOX) is loaded into the 3D printed porous DN hydrogels and the in vitro release study displays the sustainable drug release behavior. And the DOX release from hydrogel scaffold can be adjusted by the pH value of release environment. All of the results indicate that the porous DN CS/PVA hydrogel scaffolds have great application potential for tissue regeneration.
在这项工作中，设计了一种由壳聚糖 (CS) 和聚乙烯醇 (PVA) 作为骨架基质的 3D 打印双网络 (DN) 水凝胶支架。PVA 的加入明显提高了基于 CS 的水凝胶的机械性能并降低了水凝胶的溶胀行为。京尼平交联 CS 可以进行预交联，以提高水凝胶前体的粘度和 3D 可打印性，而增加 PVA 含量则降低了预交联水凝胶的粘度和 3D 可打印性。DN 水凝胶的抗菌性能结果表明，水凝胶具有良好的持久抗菌能力。适当的预交联水凝胶，CS/PVA 质量比为 3:10，预交联时间为 7 h，用于 3D 打印，制备 3D 打印多孔 DN 水凝胶。此外，将抗肿瘤药物阿霉素 (DOX) 载入 3D 打印多孔 DN 水凝胶中，并进行体外释放研究，显示出持续的药物释放行为。并且水凝胶支架中 DOX 的释放可以通过释放环境的 pH 值进行调节。所有结果表明，多孔 DN CS/PVA 水凝胶支架在组织再生方面具有巨大的应用潜力。
