School of Pharmacy, Nanjing Medical University, Nanjing City 210029, P.R. China.
ACS Appl Mater Interfaces. 2011 Sep;3(9):3308-15. doi: 10.1021/am2007855. Epub 2011 Sep 7.
In this paper, highly selective core-shell molecularly imprinted polymers (MIPs) of tadalafil on the surface of magnetic nanoparticles (MNPs) were prepared. Three widely used functional monomers 2-(trifluoromethyl) acrylic acid (TFMAA), acrylic acid (AA), and methacrylic acid (MAA) were compared theoretically as the candidates for MIP preparation. MIP-coated magnetic nanoparticles (MIP-coated MNPs) showed large adsorption capacity, high recognition ability, and fast binding kinetics for tadalafil. Furthermore, because of the good magnetic properties, MIP-coated MNPs can achieve rapid and efficient separation with an external magnetic field simply. The resulting MIP-coated MNPs were used as dispersive solid-phase extraction (DSPE) materials coupled with HPLC-UV for the selective extraction and detection of tadalafil from medicines (herbal sexual health products). Encouraging results were obtained. The amounts of tadalafil that were detected from the herbal sexual health product was 43.46 nmol g(-1), and the recoveries were in the range of 87.36-90.93% with the RSD < 6.55%.
本文制备了在磁性纳米粒子（MNPs）表面的西地那非的高选择性核壳分子印迹聚合物（MIPs）。三种广泛使用的功能单体 2-（三氟甲基）丙烯酸（TFMAA）、丙烯酸（AA）和甲基丙烯酸（MAA）被理论上作为 MIP 制备的候选物进行了比较。MIP 包覆的磁性纳米粒子（MIP-coated MNPs）对西地那非表现出大的吸附容量、高识别能力和快速的结合动力学。此外，由于良好的磁性，MIP-coated MNPs 可以在外磁场的作用下简单地实现快速和有效的分离。所得的 MIP-coated MNPs 被用作与 HPLC-UV 结合的分散固相萃取（DSPE）材料，用于从药物（草药性健康产品）中选择性提取和检测西地那非。得到了令人鼓舞的结果。从草药性健康产品中检测到的西地那非的量为 43.46 nmol g(-1)，回收率在 87.36-90.93%范围内，RSD < 6.55%。
