Cancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People's Hospital(Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China.
Department of Ultrasound, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
Acta Diabetol. 2023 Dec;60(12):1709-1718. doi: 10.1007/s00592-023-02165-1. Epub 2023 Jul 31.
To investigate the associations of GCKR and ADIPOQ variants with the risk of gestational diabetes mellitus (GDM) in Chinese women.
GCKR rs1260326, ADIPOQ rs266729, and rs1501299 were selected and genotyped in 519 GDM patients and 498 controls. Candidate SNPs were genotyped using multiplex polymerase chain reaction (PCR) combined with next-generation sequencing methods, and the association of these SNPs with GDM was analyzed.
We found that GCKR rs1260326 was significantly associated with an increased risk of GDM in the allele model, the codominant model (CC vs. TT), the dominant model, the recessive model, and the genotypic model distributions (p = 0.0029, p = 0.0022, p = 0.0402, p = 0.0038, and p = 0.0028, respectively). The rs1260326 polymorphism was shown to be associated with 1 h-OGTT level and gravidity in GDM patients (CC vs. TT: p = 0.0475 and p = 0.0220, respectively). Diastolic blood pressure (DBP) was significantly higher in the GDM patients with the rs266729 GG genotype compared to those with the CC or CG genotype (p = 0.0444 and p = 0.0339, respectively). The DBP of the GDM patients with the rs1501299 GT genotype was lower than that of those with the GG genotype (p = 0.0197). There was a weak linkage disequilibrium value between the GCKR and ADIPOQ SNPs.
The genes GCKR and ADIPOQ may be involved in the pathophysiology of GDM.
探讨葡萄糖激酶调节基因（GCKR）和脂联素（ADIPOQ）变异与中国女性妊娠期糖尿病（GDM）风险的关系。
选择葡萄糖激酶调节基因 rs1260326、ADIPOQ rs266729 和 rs1501299 进行基因分型，共纳入 519 例 GDM 患者和 498 例对照。候选 SNP 采用多重聚合酶链反应（PCR）结合下一代测序方法进行基因分型，并分析这些 SNP 与 GDM 的相关性。
我们发现 GCKR rs1260326 等位基因模型、共显性模型（CC 与 TT）、显性模型、隐性模型和基因型模型分布均与 GDM 风险增加显著相关（p=0.0029、p=0.0022、p=0.0402、p=0.0038 和 p=0.0028）。rs1260326 多态性与 GDM 患者 1 h-OGTT 水平和孕次有关（CC 与 TT：p=0.0475 和 p=0.0220）。与 CC 或 CG 基因型相比，rs266729 GG 基因型的 GDM 患者舒张压（DBP）明显更高（p=0.0444 和 p=0.0339）。GDM 患者 rs1501299 GT 基因型的 DBP 低于 GG 基因型（p=0.0197）。GCKR 和 ADIPOQ SNP 之间存在较弱的连锁不平衡值。
GCKR 和 ADIPOQ 基因可能参与了 GDM 的病理生理过程。
