Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Biochem Biophys Res Commun. 2011 Sep 16;413(1):10-6. doi: 10.1016/j.bbrc.2011.08.015. Epub 2011 Aug 19.
Drought is a major environmental factor that limits plant growth and productivity. Polyamines have been shown to act as stress molecules that accumulate in plant adaptation to abiotic stresses. In this study, an arginine decarboxylase gene isolated from Poncirus trifoliata, PtADC, was introduced into tobacco and tomato to investigate its function in drought tolerance. We demonstrate that the transgenic plants showed an improvement in dehydration and drought tolerance. Under dehydration stress conditions, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines as compared with the wild type. Moreover, the transcript levels of three stress-responsive genes were increased in the transgenic tobacco lines. Taken together, our results suggest that PtADC plays a key role in drought tolerance, which is, at least partially, attributed to its role in ROS detoxification.
干旱是限制植物生长和生产力的主要环境因素。多胺已被证明作为应激分子在植物适应非生物胁迫中积累。在这项研究中，从枳属中分离得到的一个精氨酸脱羧酶基因 PtADC 被导入烟草和番茄中，以研究其在耐旱性中的功能。我们证明，转基因植物在脱水和耐旱性方面表现出改善。在脱水胁迫条件下，与野生型相比，转基因系中活性氧（ROS）的积累显著减少。此外，在转基因烟草系中，三个应激响应基因的转录水平增加。总之，我们的结果表明 PtADC 在耐旱性中起着关键作用，至少部分归因于其在 ROS 解毒中的作用。
