Aduse Poku Samuel, Nkachukwu Chukwurah Peter, Aung Htut Htet, Nakamura Ikuo
Laboratory of Plant Cell Technology, Chiba University, 648 Matsudo, Chiba 271-8510, Japan.
Plants (Basel). 2020 Dec 10;9(12):1749. doi: 10.3390/plants9121749.
Climate change, with its attendant negative effects, is expected to hamper agricultural production in the coming years. To counteract these negative effects, breeding of environmentally resilient plants via conventional means and genetic engineering is necessary. Stress defense genes are valuable tools by which this can be achieved. Here we report the successful cloning and functional characterization of a melon Y3SK2-type dehydrin gene, designated as . We generated overexpressing transgenic tobacco lines and performed in vitro and in vivo drought and salt stress analyses. Seeds of transgenic tobacco plants grown on 10% polyethylene glycol (PEG) showed significantly higher germination rates relative to wild-type seeds. In the same way, transgenic seeds grown on 150 mM sodium chloride (NaCl) recorded significantly higher germination percentages compared with wild-type plants. The fresh weights and root lengths of young transgenic plants subjected to drought stress were significantly higher than that of wild-type plants. Similarly, the fresh weights and root lengths of transgenic seedlings subjected to salt stress treatments were also significantly higher than wild-type plants. Moreover, transgenic plants subjected to drought and salt stresses in vivo showed fewer signs of wilting and chlorosis, respectively. Biochemical assays revealed that transgenic plants accumulated more proline and less malondialdehyde (MDA) compared with wild-type plants under both drought and salt stress conditions. Finally, the enzymatic activities of ascorbate peroxidase (APX) and catalase (CAT) were enhanced in drought- and salt-stressed transgenic lines. These results suggest that the gene could be used as a potential candidate gene for crop improvement.
气候变化及其带来的负面影响预计将在未来几年阻碍农业生产。为了抵消这些负面影响，通过传统方法和基因工程培育具有环境适应能力的植物是必要的。应激防御基因是实现这一目标的宝贵工具。在此，我们报告了一个甜瓜Y3SK2型脱水素基因的成功克隆和功能表征，该基因命名为 。我们构建了过表达该基因的转基因烟草株系，并进行了体外和体内的干旱和盐胁迫分析。在10%聚乙二醇（PEG）上生长的转基因烟草种子的发芽率相对于野生型种子显著更高。同样，在150 mM氯化钠（NaCl）上生长的转基因种子的发芽率与野生型植株相比也显著更高。遭受干旱胁迫的转基因幼苗的鲜重和根长显著高于野生型植株。同样，遭受盐胁迫处理的转基因幼苗的鲜重和根长也显著高于野生型植株。此外，在体内遭受干旱和盐胁迫的转基因植株分别表现出较少的萎蔫和黄化迹象。生化分析表明，在干旱和盐胁迫条件下，与野生型植株相比，转基因植株积累了更多的脯氨酸和更少的丙二醛（MDA）。最后，在干旱和盐胁迫的转基因株系中，抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）的酶活性增强。这些结果表明，该 基因可作为作物改良的潜在候选基因。
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