Department of Analytical Chemistry, Annex C-3, Campus of Rabanales, University of Córdoba, E-14071, Córdoba, Spain.
Talanta. 2011 Sep 30;85(4):1842-7. doi: 10.1016/j.talanta.2011.07.031. Epub 2011 Jul 18.
The automated method developed for the determination of carotenoids uses 200 μL of serum, which was mixed with 400 μL of tetrahydrofuran, vortexed for 1 min, settled for 10 min, centrifuged for 6 min and the supernatant injected into an automatic solid-phase extraction (SPE) system for cleanup-preconcentration. A 10% water-acetonitrile mobile phase at 1.5 mL min(-1) eluted the retained compounds and transferred them on-line to a reversed-phase analytical column for individual separation of the target analytes. Visible detection was performed at 450 and 460 nm. The detection limits for the target analytes were between 3 and 30 ng mL(-1); the precision (expressed as relative standard deviation) ranged between 2.83 and 5.06% for repeatability and between 3.80 and 7.40% for within laboratory reproducibility. The total analysis time was 18 min. The proposed method is reliable, robust, and has an excellent potential for high-throughput use in both clinical and research laboratories.
本研究建立的自动化方法用于测定类胡萝卜素，需取 200 μL 血清与 400 μL 四氢呋喃混合，涡旋 1 分钟，沉淀 10 分钟，离心 6 分钟，上清液注入自动固相萃取（SPE）系统进行净化-预浓缩。采用 10%水-乙腈（1.5 mL min(-1)）作为流动相洗脱保留的化合物，并在线转移到反相分析柱上，对目标分析物进行单独分离。可见检测波长为 450nm 和 460nm。目标分析物的检测限在 3 至 30ng mL(-1)之间；精密度（以相对标准偏差表示）重复性为 2.83%至 5.06%，实验室内重现性为 3.80%至 7.40%。总分析时间为 18 分钟。该方法可靠、稳健，具有高通量应用于临床和研究实验室的巨大潜力。
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