Department of Surgery, St. Luke's-Roosevelt Hospital Center, Columbia University College of Physicians and Surgeons, New York, New York 10019, USA.
J Surg Res. 2011 Nov;171(1):e113-21. doi: 10.1016/j.jss.2011.06.017. Epub 2011 Jul 7.
The hallmark of lung ischemia-reperfusion injury (IRI) is the production of reactive oxygen species (ROS), and the resultant oxidant stress has been implicated in apoptotic cell death as well as subsequent development of inflammation. Dietary flaxseed (FS) is a rich source of naturally occurring antioxidants and has been shown to reduce lung IRI in mice. However, the mechanisms underlying the protective effects of FS in IRI remain to be determined.
We used a mouse model of IRI with 60 min of ischemia followed by 180 min of reperfusion and evaluated the anti-apoptotic and anti-inflammatory effects of 10% FS dietary supplementation.
Mice fed 10% FS undergoing lung IRI had significantly lower levels of caspases and decreased apoptotic activity compared with mice fed 0% FS. Lung homogenates and bronchoalveolar lavage fluid analysis demonstrated significantly reduced inflammatory infiltrate in mice fed with 10% FS diet. Additionally, 10% FS treated mice showed significantly increased expression of antioxidant enzymes and decreased markers of lung injury.
We conclude that dietary FS is protective against lung IRI in a clinically relevant murine model, and this protective effect may in part be mediated by the inhibition of apoptosis and inflammation.
肺缺血再灌注损伤 (IRI) 的标志是活性氧 (ROS) 的产生，由此产生的氧化应激与细胞凋亡以及随后的炎症发展有关。食用亚麻籽 (FS) 是天然抗氧化剂的丰富来源，已被证明可减少小鼠的肺 IRI。然而，FS 在 IRI 中的保护作用的机制仍有待确定。
我们使用了一种 60 分钟缺血后再灌注 180 分钟的 IRI 小鼠模型，并评估了 10%FS 饮食补充对抗细胞凋亡和抗炎的作用。
与喂食 0%FS 的小鼠相比，接受肺 IRI 的喂食 10%FS 的小鼠的半胱天冬酶水平明显更低，凋亡活性降低。肺匀浆和支气管肺泡灌洗液分析表明，喂食 10%FS 饮食的小鼠中的炎性浸润明显减少。此外，10%FS 治疗的小鼠表现出抗氧化酶表达显著增加和肺损伤标志物减少。
我们得出结论，饮食 FS 在临床相关的小鼠模型中对肺 IRI 具有保护作用，这种保护作用可能部分是通过抑制细胞凋亡和炎症来介导的。
