Sohrabipour Shahla, Jafari Adele, Kamalinejad Mohamad, Sarrafnejd Abdolfatah, Shahrestany Taherah, Sadeghipour Hamid-Reza
Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Reprod Med. 2013 Jun;11(6):459-66.
Prepubertal varicocele has the most devastating effects on the testes. Oxidative stress is the major cause leading to infertility in varicocele. The antioxidant properties of Flaxseed (FS) treatment in some oxidative diseases have been reported.
This study aimed to evaluate the antioxidant effect of FS in prepubertal rats with experimental varicocele.
Forty two male prepubertal rats were divided into 6 groups: the varicocele group were either fed with 10% FS, or with regular diet, or with Vit E, the group with sham operation fed with 10% FS, or had regular diet, and control rats who had not been operated but received regular diet. Varicocele was created by Koksal method. After 6 weeks sperm superoxide anion and H2O2 were evaluated by flowcytometery. Semen total antioxidant capacity (TAC) by Koracevic method and testes malondialdehyde (MDA) by thiobarbituric acid with spectrophotometry was measured.
While superoxide anion and H2O2 were significantly higher in varicocele grop with regular diet (p=0.0001), FS significantly decreased the previously-mentioned parameters (p=0.0001). There were no significant differences for seminal TAC between 6 groups (p=0.07). Left testicular MDA concentration were lower in varicocele or group that were fed with 10% FS compared with other groups (p=0.001).
Reactive oxygen species (ROS) may cause sperm oxidative damage. FS as a fat soluble antioxidant can scavenge intracellular ROS production in varicocele.
青春期前精索静脉曲张对睾丸的影响最为严重。氧化应激是导致精索静脉曲张患者不孕的主要原因。已有报道称亚麻籽（FS）治疗在某些氧化疾病中具有抗氧化特性。
本研究旨在评估FS对青春期前实验性精索静脉曲张大鼠的抗氧化作用。
42只青春期前雄性大鼠分为6组：精索静脉曲张组分别给予10%FS、常规饮食或维生素E；假手术组分别给予10%FS或常规饮食；对照组未进行手术但给予常规饮食。采用科萨尔方法制造精索静脉曲张。6周后，通过流式细胞术评估精子超氧阴离子和过氧化氢。采用科拉切维奇方法测定精液总抗氧化能力（TAC），采用硫代巴比妥酸分光光度法测定睾丸丙二醛（MDA）。
常规饮食的精索静脉曲张组超氧阴离子和过氧化氢显著升高（p = 0.0001），而FS显著降低了上述参数（p = 0.0001）。6组精液TAC无显著差异（p = 0.07）。与其他组相比，给予10%FS的精索静脉曲张组或该组左侧睾丸MDA浓度较低（p = 0.001）。
活性氧（ROS）可能导致精子氧化损伤。FS作为一种脂溶性抗氧化剂，可清除精索静脉曲张时细胞内ROS的产生。
