Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis & Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China.
Biosens Bioelectron. 2011 Nov 15;29(1):109-14. doi: 10.1016/j.bios.2011.08.002. Epub 2011 Aug 6.
A colorimetric and luminescent bifunctional Ru(II) complex-modified gold nano-probe for sensing ctDNA was developed. A new water-soluble Ru(II) complex containing an aromatic α-diimine ligand with an extended π system was found to be emissive at about 588nm in water and the emission intensity of the complex was enhanced about 3.4-fold upon addition of calf thymus DNA (ctDNA) in aqueous buffer with no color changes. The detection limit was 70nM with a 1.2% relative standard deviation (RSD, n=5). The Ru(II) complex chromophore was used to modify 8nm MHA carboxylate-functionalized gold nanoparticles to produce a colorimetric and luminescent bifunctional probe for sensing ctDNA in aqueous buffer at room temperature. The obvious change in visible color and enhancement of emission intensity of the functionalized gold-Ru(II) complex colloids upon addition of ctDNA was due to the electron or energy transfer between Ru(II) chromophore and the Au-NPs. The limit of detection was 1.0nM for ctDNA with a 4.5% RSD (n=5). With such a high sensitivity, the bifunctional Ru(II) complex-modified gold nano-probe will be potentially suitable for the DNA sensing in bioanalytical application.
一种用于检测 ctDNA 的比率型比色和荧光双功能 Ru(II) 配合物修饰的金纳米探针被开发出来。研究发现，一种新的水溶性 Ru(II) 配合物，含有一个具有扩展π体系的芳香α-二亚胺配体，在水中约 588nm 处具有发光性，当在含有小牛胸腺 DNA(ctDNA)的水溶液缓冲液中加入 ctDNA 时，其发射强度增强了约 3.4 倍，而没有颜色变化。检测限为 70nM，相对标准偏差(RSD)为 1.2%（n=5）。该 Ru(II) 配合物发色团被用于修饰 8nm MHA 羧酸功能化的金纳米粒子，以在室温下的水溶液缓冲液中产生用于检测 ctDNA 的比率型比色和荧光双功能探针。由于 Ru(II) 发色团和 Au-NPs 之间的电子或能量转移，功能化金-Ru(II) 配合物胶体在加入 ctDNA 后可见颜色明显变化和荧光强度增强。ctDNA 的检测限为 1.0nM，RSD 为 4.5%（n=5）。如此高的灵敏度，使双功能 Ru(II) 配合物修饰的金纳米探针将有望适用于生物分析应用中的 DNA 传感。
