Key Laboratory for Analytical Science of Food Safety and Biology (Ministry of Education), Key Laboratory of Analysis and Detection Technology for Food Safety (Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350116, P. R. China.
Analyst. 2017 Oct 7;142(19):3733-3739. doi: 10.1039/c7an01253e. Epub 2017 Sep 5.
A sensitive assay was demonstrated for the colorimetric and luminescent detection of heparin. Positively charged [Ru(phen)np] (np: naphtho[2,3-f][1,10]phenanthroline) can interact with negatively charged AuNPs through electrostatic interaction, leading to the luminescence quenching of [Ru(phen)np]via energy transfer as well as the aggregation of AuNPs together with a color change in solution from red to blue. Upon the addition of negatively charged heparin to gold nanoparticles functionalized by ruthenium(ii) complexes, the emission of [Ru(phen)np] recovered due to the competitive adsorption of [Ru(phen)np] to heparin and AuNPs. A linear calibration curve was obtained in the 0.4 μM to 100 μM range and 0.1 μM to 6 μM with a low detection limit of 0.22 μM and 0.024 μM based on 3σ/s for heparin quantification in buffer solution and in 1% fetal bovine serum (FBS), respectively. Interestingly, the naked eye alone can judge the presence of 0.02 μM heparin without the aid of any advanced instruments by color change. Thus a convenient and visual method to detect heparin was established.
一种灵敏的比色和荧光检测肝素的方法得到了证明。带正电荷的[Ru(phen)np]（np：萘并[2,3-f][1,10]菲咯啉）可以通过静电相互作用与带负电荷的 AuNPs 相互作用，导致[Ru(phen)np]通过能量转移而猝灭，以及 AuNPs 的聚集以及溶液颜色从红色变为蓝色。当带负电荷的肝素被加入到金纳米粒子功能化的钌(ii)配合物时，由于[Ru(phen)np]与肝素和 AuNPs 的竞争吸附，[Ru(phen)np]的发射恢复。在缓冲溶液中和 1%胎牛血清（FBS）中，分别在 0.4 μM 至 100 μM 范围内和 0.1 μM 至 6 μM 范围内获得了线性校准曲线，检测限分别为 0.22 μM 和 0.024 μM，基于 3σ/s 进行肝素定量。有趣的是，仅通过肉眼就可以通过颜色变化判断 0.02 μM 肝素的存在，而无需任何先进仪器的帮助。因此，建立了一种方便和可视化的检测肝素的方法。
