National Poison Centre, Universiti Sains Malaysia, 11800 Penang, Malaysia.
J Chromatogr A. 2011 Sep 28;1218(39):7055-60. doi: 10.1016/j.chroma.2011.08.037. Epub 2011 Aug 17.
Sildenafil analogues have been found adulterated in herbal preparations and food products that claim to have natural aphrodisiacs. In this study, a gas chromatography-mass spectrometry (GC-MS) assay was developed for the screening and identification of thioketone analogues of sildenafil. Thiopyrazolopyrimidine, a precursor or a cleavage product of thioketone analogue, exhibited characteristic fragment ions of m/z 328 and m/z 299 was found to be the best marker to screen the presence of general thioketone analogues. Identification by GC-MS assay was rapid and specific as all the studied thioketones showed characteristic mass fragmentations including their intact molecular ions. The developed GC-MS assay had successfully identified thiosildenafil, thiohomosildenafil and thiodimethylsildenafil in herbal preparation and food products.
西地那非类似物已在声称具有天然壮阳作用的草药制剂和食品中被发现掺假。在这项研究中，开发了一种气相色谱-质谱（GC-MS）测定法，用于筛查和鉴定西地那非的硫酮类似物。噻吩并吡唑嘧啶，硫酮类似物的前体或裂解产物，表现出特征碎片离子 m/z 328 和 m/z 299，被发现是筛查一般硫酮类似物存在的最佳标记物。GC-MS 测定法的鉴定快速且具有特异性，因为所有研究的硫酮都表现出包括其完整分子离子在内的特征质谱裂解。所开发的 GC-MS 测定法已成功地在草药制剂和食品中鉴定出硫代西地那非、硫代homosildenafil 和硫代二甲基西地那非。
