Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
J Hazard Mater. 2011 Nov 15;195:230-7. doi: 10.1016/j.jhazmat.2011.08.034. Epub 2011 Aug 17.
Among a collection of Ni resistant endophytes isolated from the tissues of Alyssum serpyllifolium, four plant growth promoting endophytic bacteria (PGPE) were selected based on their ability to promote seedling growth in roll towel assay. Further, the PGPE screened showed the potential to produce plant growth promoting (PGP) substances and plant polymer hydrolyzing enzymes. These isolates were further screened for their PGP activity on A. serpyllifolium and Brassica juncea under Ni stress using a phytagar assay. None of the four isolates produced any disease symptoms in either plant. Further, strain A3R3 induced a maximum increase in biomass and Ni content of plants. Based on the PGP potential in phytagar assay, strain A3R3 was chosen for studying its PGP effect on A. serpyllifolium and B. juncea in Ni contaminated soil. Inoculation with A3R3 significantly increased the biomass (B. juncea) and Ni content (A. serpyllifolium) of plants grown in Ni contaminated soil. The strain also showed high level of colonization in tissue interior of both plants. By 16S rRNA gene sequencing analysis, A3R3 was identified as Pseudomonas sp. Successful colonization and subsequent PGP potentiality of Pseudomonas sp. A3R3 indicate that the inoculation with PGPE might have significant potential to improve heavy metal phytoremediation.
从 Alyssum serpyllifolium 组织中分离出的耐镍内生菌中，选择了 4 种具有促进幼苗生长能力的植物促生内生细菌（PGPE），通过滚毛巾试验进行筛选。此外，筛选出的 PGPE 具有产生植物生长促进（PGP）物质和植物聚合物水解酶的潜力。这些分离物进一步在含镍胁迫下的 Alyssum serpyllifolium 和 Brassica juncea 上通过植物琼脂试验筛选其 PGPE 活性。这 4 种分离物均未在任何一种植物上产生任何疾病症状。此外，菌株 A3R3 诱导植物生物量和镍含量最大增加。基于植物琼脂试验中的 PGP 潜力，选择菌株 A3R3 研究其在镍污染土壤中对 Alyssum serpyllifolium 和 Brassica juncea 的 PGP 效应。接种 A3R3 可显著增加在镍污染土壤中生长的植物的生物量（B. juncea）和镍含量（A. serpyllifolium）。该菌株还在两种植物的组织内部表现出高水平的定殖。通过 16S rRNA 基因测序分析，A3R3 被鉴定为假单胞菌。假单胞菌 A3R3 的成功定殖及其随后的 PGP 潜力表明，接种 PGPE 可能具有显著提高重金属植物修复的潜力。
