超声微泡介导 5-氨基酮戊酸声动力学治疗大鼠脑内深部胶质瘤

Sonodynamic therapy with 5-aminolevulinic acid and focused ultrasound for deep-seated intracranial glioma in rat.

机构信息

Department of Neurosurgery, Fukuoka University, Faculty of Medicine, 7-45-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan.

出版信息

Anticancer Res. 2011 Jul;31(7):2527-33.

PMID:21873170

Abstract

BACKGROUND

5-Aminolevulinic acid (5-ALA) has already been applied clinically as a photosensitizer. In this study, sonodynamically induced selective antitumour effect of 5-ALA for deep-seated lesions was evaluated.

MATERIALS AND METHODS

First, normal rat brains were sonicated via a transducer placed on the dural surface to confirm safe acoustic conditions for normal rat brains. One week after inoculation of brains with C6 rat glioma cells, brains with/without administration of 5-ALA (100 mg/kg body weight) were sonicated.

RESULTS

Sonodynamic therapy (SDT) with 5-ALA and focused ultrasound (10 W/cm(2), 1.04 MHz, 5 min) achieved selective antitumour effect against deep-seated experimental glioma. Mean tumour sizes in the largest coronal section in sham-operated rats and rats receiving ultrasound with/without 5-ALA were 29.94±10.39, 18.32±5.69 and 30.81±9.65 mm(2), respectively. Tumour size was significantly smaller in the SDT group than in other groups (p<0.05).

CONCLUSION

This experimental rat model showed that SDT appears to be useful in the treatment of deep-seated malignant glioma.

摘要

背景

5-氨基酮戊酸（5-ALA）已作为光敏剂在临床上得到应用。本研究评估了声动力疗法（SDT）应用 5-ALA 对深部病变的选择性抗肿瘤作用。

材料与方法

首先，通过放置在硬脑膜表面的换能器对正常大鼠的大脑进行超声处理，以确认正常大鼠大脑的安全声条件。在 C6 大鼠神经胶质瘤细胞接种后一周，对有/无 5-ALA（100mg/kg 体重）给药的大脑进行超声处理。

结果

5-ALA 和聚焦超声（10W/cm²，1.04MHz，5min）的声动力学疗法（SDT）对深部实验性脑胶质瘤具有选择性抗肿瘤作用。假手术组和接受超声联合/不联合 5-ALA 治疗的大鼠最大冠状切片中的平均肿瘤大小分别为 29.94±10.39、18.32±5.69 和 30.81±9.65mm²。与其他组相比，SDT 组的肿瘤体积明显较小（p<0.05）。