shotgun 同位素探针在微生物群落中快速、特异性检测微生物的应用。
Shotgun isotope array for rapid, substrate-specific detection of microorganisms in a microbial community.
机构信息
Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
出版信息
Appl Environ Microbiol. 2011 Oct;77(20):7430-2. doi: 10.1128/AEM.00121-11. Epub 2011 Aug 26.
Abstract
The shotgun isotope array method has been proposed to be an effective new tool for use in substrate-specific microbe exploration without any prior knowledge of the community composition. Proof of concept was demonstrated by detection of acetate-degrading microorganisms in activated sludge and further verified by independent stable isotope probing (SIP).
摘要
shotgun 同位素探针法被提议作为一种新的有效工具，用于在没有任何群落组成先验知识的情况下进行特定基质微生物的探测。通过在活性污泥中检测乙酸降解微生物，证明了该方法的概念验证，并且通过独立的稳定同位素探针（SIP）进一步验证。
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