氯胺酮、水合氯醛和戊巴比妥钠麻醉对离体大鼠心脏和心肌细胞影响的比较。
A comparison of the effects of ketamine, chloral hydrate and pentobarbital sodium anesthesia on isolated rat hearts and cardiomyocytes.
机构信息
Department of Physiology, Xuzhou Medical College, Xuzhou, China.
出版信息
J Cardiovasc Med (Hagerstown). 2011 Oct;12(10):732-5. doi: 10.2459/JCM.0b013e32834a6697.
OBJECTIVES
The study was intended to investigate which commonly used anesthetic in intact animals has the least effect on the function of isolated hearts and cardiomyocytes among the anesthetized animals.
METHODS
The hearts of male Sprague-Dawley rats were removed after they were anesthetized with ketamine, chloral hydrate or pentobarbital sodium, respectively, or were cervically dislocated. They were mounted on a Langendorff shelf. Heart rate (HR), left ventricular systolic pressure (LVSP), and maximal rate of increase of left ventricular pressure (+dp/dt) were observed and recorded. Cell shorting amplitude and survival rate were detected in isolated cardiomyocytes.
RESULTS
The application of ketamine and pentobarbital sodium led to a significant decrease in HR, LVSP and +dp/dt in isolated hearts. Furthermore, pentobarbital sodium inhibited cell shorting amplitude and reduced the survival rate of isolated cardiomyocytes. Chloral hydrate did not significantly alter HR, LVSP, +dp/dt, cell shorting amplitude and survival rate.
CONCLUSION
The effects of anesthetics on cardiac parameters were considered when choosing an anesthesia administration. The results suggested that chloral hydrate as an anesthetic was appropriately applied for the studies of isolated hearts and cardiomyocytes.
目的
本研究旨在探究在麻醉动物中，哪种常用的麻醉剂对分离心脏和心肌细胞的功能影响最小。
方法
雄性 Sprague-Dawley 大鼠在使用氯胺酮、水合氯醛或戊巴比妥钠麻醉后或颈椎脱位后，取出心脏，分别置于 Langendorff 支架上。观察并记录心率（HR）、左心室收缩压（LVSP）和左心室压力最大上升速率（+dp/dt）。检测分离的心肌细胞的短缩幅度和存活率。
结果
氯胺酮和戊巴比妥钠的应用导致分离心脏的 HR、LVSP 和+dp/dt 显著下降。此外，戊巴比妥钠抑制细胞短缩幅度并降低分离心肌细胞的存活率。水合氯醛对 HR、LVSP、+dp/dt、细胞短缩幅度和存活率无显著影响。
结论
在选择麻醉给药方式时，应考虑麻醉剂对心脏参数的影响。结果表明，水合氯醛作为一种麻醉剂，适用于分离心脏和心肌细胞的研究。
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