Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Nat Methods. 2011 Aug 28;8(10):845-7. doi: 10.1038/nmeth.1675.
We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.
我们通过喂食重同位素标记的大肠杆菌来证明秀丽隐杆线虫的赖氨酸的重同位素标记。使用重同位素标记的线虫和定量蛋白质组学方法，我们鉴定了几种在秀丽隐杆线虫中响应核激素受体 49 的缺失或 RNAi 介导的敲低而被调控的蛋白质。定量蛋白质组学和选择性基因敲低的结合使用是秀丽隐杆线虫生物学的有力工具。
