Singh Kapil Dev, Zheng Xue, Milstein Stuart, Keller Martin, Roschitzki Bernd, Grossmann Jonas, Hengartner Michael O
Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
PhD Program in Molecular Life Science, University of Zurich and ETH Zurich, Zurich, Switzerland.
PLoS One. 2017 Jul 31;12(7):e0182270. doi: 10.1371/journal.pone.0182270. eCollection 2017.
Cytoplasmic polyadenylation element binding (CPEB) proteins are evolutionary conserved RNA-binding proteins that control mRNA polyadenylation and translation. Orthologs in humans and other vertebrates are mainly involved in oogenesis. This is also the case for the C. elegans CPEB family member CPB-3, whereas two further CPEB proteins (CPB-1 and FOG-1) are involved in spermatogenesis. Here we describe the characterisation of a new missense allele of cpb-3 and show that loss of cpb-3 function leads to an increase in physiological germ cell death. To better understand the interaction and effect of C. elegans CPEB proteins on processes such as physiological apoptosis, germ cell differentiation, and regulation of gene expression, we characterised changes in the transcriptome and proteome of C. elegans CPEB mutants. Our results show that, despite their sequence similarities CPEB family members tend to have distinct overall effects on gene expression (both at the transcript and protein levels). This observation is consistent with the distinct phenotypes observed in the various CPEB family mutants.
细胞质聚腺苷酸化元件结合（CPEB）蛋白是进化上保守的RNA结合蛋白，可控制mRNA的聚腺苷酸化和翻译。人类和其他脊椎动物中的直系同源物主要参与卵子发生。秀丽隐杆线虫的CPEB家族成员CPB - 3也是如此，而另外两种CPEB蛋白（CPB - 1和FOG - 1）则参与精子发生。在此，我们描述了cpb - 3一个新的错义等位基因的特征，并表明cpb - 3功能丧失会导致生理性生殖细胞死亡增加。为了更好地理解秀丽隐杆线虫CPEB蛋白在生理性凋亡、生殖细胞分化和基因表达调控等过程中的相互作用和影响，我们对秀丽隐杆线虫CPEB突变体的转录组和蛋白质组变化进行了表征。我们的结果表明，尽管CPEB家族成员在序列上具有相似性，但它们对基因表达（在转录和蛋白质水平上）往往具有不同的总体影响。这一观察结果与在各种CPEB家族突变体中观察到的不同表型一致。
