Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47., POB 260, Budapest, 1444, Hungary.
Inflamm Res. 2011 Nov;60(11):1061-70. doi: 10.1007/s00011-011-0367-9. Epub 2011 Aug 27.
The role of NO and adipocytokines in childhood obesity was studied, supposing that obesity provokes inflammation. Children were admitted to the pediatric clinic for a regular check up because of obesity.
Obese (n = 79) and healthy (n = 12) children were selected and divided into subgroups according to their age, gender, glucose tolerance and nitric oxide synthase (NOS II) positivity.
Urine and blood nitrite plus nitrate, the expression of NOS II in white blood cells, serum adipocytokines and clinical characteristics were analyzed in each group. Significance was tested by unpaired two-tailed t test and by ANOVA.
NOS II was only detected in the white blood cells of a subgroup (17/79) of obese children. Serum leptin and resistin concentrations were significantly higher, adiponectin was lower compared to healthy children. Significant correlations were observed between serum adiponectin and resistin levels (reciprocal, R (2) = 0.4), and between body mass index and serum leptin levels.
NOS II expression in white blood cells was observed in a minority of patients. Low-grade inflammation in obese children was suggested by the increased resistin levels, particularly in NOS II-positive patients. Correlation between different adipocytokines was restricted for a few subgroups.
研究了 NO 和脂肪细胞因子在儿童肥胖中的作用，假设肥胖会引发炎症。由于肥胖，儿童被送到儿科诊所进行常规检查。
选择了肥胖（n=79）和健康（n=12）儿童，并根据年龄、性别、葡萄糖耐量和一氧化氮合酶（NOS II）阳性情况将其分为亚组。
分析了每组儿童的尿液和血液中亚硝酸盐/硝酸盐、白细胞中 NOS II 的表达、血清脂肪细胞因子和临床特征。采用非配对双尾 t 检验和方差分析进行检验。
仅在肥胖儿童的一个亚组（17/79）的白细胞中检测到 NOS II。与健康儿童相比，血清瘦素和抵抗素浓度显著升高，脂联素水平降低。血清脂联素与抵抗素水平之间存在显著相关性（倒数，R（2）=0.4），并且体重指数与血清瘦素水平之间存在相关性。
在少数患者中观察到白细胞中 NOS II 的表达。肥胖儿童的低度炎症由抵抗素水平升高引起，尤其是在 NOS II 阳性患者中。不同脂肪细胞因子之间的相关性仅限于少数亚组。
