对显示出对单端孢霉烯族抗生素具有高水平或低水平抗性的酿酒酵母菌株的特性分析。
Characterization of Saccharomyces cerevisiae strains displaying high-level or low-level resistance to trichothecene antibiotics.
作者信息
Fernandez-Lobato M, Cannon M, Mitlin J A, Mount R C, Jimenez A
机构信息
Centro de Biologia Molecular, CSIC, Universidad Autonoma de Madrid, Spain.
出版信息
Biochem J. 1990 May 1;267(3):709-13. doi: 10.1042/bj2670709.
Abstract
Biochemical and genetic analyses have been carried out on Saccharomyces cerevisiae strains characterized in vivo as sensitive, low-level-resistant or high-level-resistant to trichothecene antibiotics. Levels of drug resistance in vitro were determined for each strain and for suitable diploids derived from them. Ribosome biogenesis was also studied in selected haploids. It is suggested that resistance in all cases results from a mutation in the gene encoding ribosomal protein L3. If this is indeed the situation, then different mutations in this same gene not only can cause low-level or high-level resistance to trichothecene antibiotics but also can affect the maturation of either 40 S or 60 S ribosomal subunits.
摘要
已对酿酒酵母菌株进行了生化和遗传分析，这些菌株在体内表现为对单端孢霉烯族抗生素敏感、低水平抗性或高水平抗性。测定了每个菌株及其衍生的合适二倍体的体外耐药水平。还对选定的单倍体进行了核糖体生物发生研究。研究表明，所有情况下的抗性均源于编码核糖体蛋白L3的基因突变。如果确实如此，那么同一基因中的不同突变不仅会导致对单端孢霉烯族抗生素的低水平或高水平抗性，还会影响40 S或60 S核糖体亚基的成熟。
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本文引用的文献
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2
Maturation of ribosomal precursor RNA in Saccharomyces cerevisiae. A mutant with a defect in both the transport and terminal processing of the 20 S species.酿酒酵母中核糖体前体RNA的成熟。一个在20S种类的转运和末端加工方面都有缺陷的突变体。
J Mol Biol. 1980 Oct 25;143(2):179-99. doi: 10.1016/0022-2836(80)90197-7.
3
A trichodermin-resistant mutant of Saccharomyces cerevisiae with an abnormal distribution of native ribosomal subunits.一株酿酒酵母的抗木霉菌素突变体，其天然核糖体亚基分布异常。
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4
Studies on the structure and biogenesis of yeast ribosomes.酵母核糖体的结构与生物发生研究。
Biochem Soc Symp. 1982;47:79-93.
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10
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J Mol Biol. 1972 Mar 28;65(2):227-42. doi: 10.1016/0022-2836(72)90279-3.
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