Giantsos Kristina, Cluff Mark, Dull Randal
Department of Anesthesiology, University of Utah School of Medicine, Salt Lake City, UT, USA.
Methods Mol Biol. 2011;763:291-301. doi: 10.1007/978-1-61779-191-8_20.
Alterations in endothelial permeability are a hallmark of inflammation as well as the underlying cause of many clinical syndromes. Quantifying changes in endothelial barrier properties to water and macromolecules can be an important means of assessing the degree of cellular injury and, conversely, the effect of therapies to attenuate the inflammatory cascade. We use a combination of an isolated organ system and two cell culture models to investigate mechanisms of endothelial barrier regulation under variety of experimental conditions. Each assay has its own experimental strengths and limitations and must be used appropriately for the questions being asked. When used collectively, they can provide significant insight into the molecular regulation of lung endothelial permeability.
内皮细胞通透性的改变是炎症的一个标志，也是许多临床综合征的潜在原因。量化内皮屏障对水和大分子的特性变化，可能是评估细胞损伤程度的重要手段，反之，也是评估减轻炎症级联反应疗法效果的重要手段。我们使用一个离体器官系统和两种细胞培养模型相结合的方法，来研究在各种实验条件下内皮屏障调节的机制。每种检测方法都有其自身的实验优势和局限性，必须根据所提出的问题适当地使用。当综合使用时，它们可以为肺内皮细胞通透性的分子调节提供重要的见解。
