Department of Pathology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Virchows Arch. 2011 Oct;459(4):361-6. doi: 10.1007/s00428-011-1140-8. Epub 2011 Aug 28.
To evaluate roles of tumor-associated macrophages (TAMs) for prognosis of classical Hodgkin lymphoma (CHL). Expression of markers for TAMs, CD68, HLA-DR, CD163, HLA-DR/CD68 (M1), and CD163/CD68 (M2) was immunohistochemically examined in 82 cases with CHL. Positively stained cells were counted and correlation of number of TAMs and patients' survival time was analyzed. Number of CD163+ cells and M2 cells was significantly correlated with shorter overall survival (P < 0.05), while it was marginally significant for CD68+ cells (P = 0.0827). HLA-DR + cells and M1 cells showed no significant correlation with overall survival. When confined to mixed cellularity subtype, number of M1 cells was correlated with favorable prognosis (P < 0.05), while M2 did not (P = 0.7). Older age and male sex were unfavorable factors for prognosis. At multivariate analysis, number of CD163+ cells, M2+ cells, and age were independent factors for poor overall survival (P = 0.03, 0.02, and 0.01, respectively). CD163+ cells and M2 cells might work to be tumor promotive in CHL. M1 cells might be tumor suppressive in mixed cellularity type.
评估肿瘤相关巨噬细胞（TAMs）在经典霍奇金淋巴瘤（CHL）预后中的作用。对 82 例 CHL 患者的 TAMs 标志物 CD68、HLA-DR、CD163、HLA-DR/CD68（M1）和 CD163/CD68（M2）进行免疫组织化学检测。计数阳性染色细胞，并分析 TAMs 数量与患者生存时间的相关性。CD163+细胞和 M2 细胞的数量与总生存率显著相关（P<0.05），而 CD68+细胞的数量则呈边缘显著相关（P=0.0827）。HLA-DR+细胞和 M1 细胞与总生存率无显著相关性。当仅限于混合细胞亚型时，M1 细胞的数量与预后良好相关（P<0.05），而 M2 则没有（P=0.7）。年龄较大和男性是预后不良的因素。多因素分析显示，CD163+细胞、M2+细胞和年龄是总生存率不良的独立因素（P=0.03、0.02 和 0.01）。CD163+细胞和 M2 细胞可能在 CHL 中发挥促进肿瘤的作用。M1 细胞可能在混合细胞型中具有肿瘤抑制作用。
