Sirica A E, Mathis G A, Sano N, Elmore L W
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond.
Pathobiology. 1990;58(1):44-64. doi: 10.1159/000163564.
Recent advances made in the isolation, culture, and transplantation of defined populations of intrahepatic biliary epithelial cells and oval cells have permitted direct analysis of the functions, growth properties, and differentiation potential of these respective cell types in their untransformed or transformed states. This review provides a current and comprehensive examination of the various approaches that have been taken to isolate and culture intrahepatic biliary epithelial cells from normal and cholestatic liver and oval cells from preneoplastic liver. Emphasis is placed on comparing the phenotypic features and growth properties of these various biliary cell types in vitro as well as on describing their transplantation into ectopic tissue sites. In addition, the oval cell is evaluated in terms of its potential role as a 'facultative stem cell' during hepatocarcinogenesis.
肝内胆管上皮细胞和卵圆细胞特定群体的分离、培养及移植方面的最新进展，使得直接分析这些各自细胞类型在未转化或转化状态下的功能、生长特性及分化潜能成为可能。本综述对从正常和胆汁淤积性肝脏中分离和培养肝内胆管上皮细胞以及从癌前肝脏中分离和培养卵圆细胞所采用的各种方法进行了全面且最新的审视。重点在于比较这些不同胆管细胞类型在体外的表型特征和生长特性，以及描述它们移植到异位组织部位的情况。此外，还对卵圆细胞在肝癌发生过程中作为“兼性干细胞”的潜在作用进行了评估。
