Wen Hong-Mei, Chang Si-Si, Lu Lin, Tian Bing-Jun, Zhao Zhi-Xian, Zhang Jie, Tang Jing-Jing, Ding Zheng-Rong
School of Public Health, Kunming Medical College, Kunming 650031, China.
Bing Du Xue Bao. 2011 Jul;27(4):342-6.
To explore the enteroviruses surveillance among healthy children under 15 years old in the border areas of Yunnan Province and Myanmar in 2009. The stool samples were collected from the healthy children under 15 years old who came from the border areas of Myanmar and Yunnan Province, virus isolation and sequencing were conducted for all the 271 samples. 6 strains of polioviruses (PVs) were detected from 271 stools with an isolation rate of 2.8%, which belonged to vaccine strains and 24 non-polioviruses (NPVs) were detected with an isolation rate of 8.9%. 24 NPVs belonged to human enterovirus group B (HEV-B) with 6 serotypes, HEV-A, HEV-C and HEV-D viruses were not isolated. Among them, 13 NPVs were E7 (54.17%) and 5 NPVs were E13 (20.83%). Our results showed that the enterovirus carrying rate in the border areas of Yunnan province was higher than the rate of routine acute flaccid paralysis (AFP) detection system. The HEV-B viruses were the only enteroviruses isolated. The phylogenetical analysis showed that Echovirus 7(E7) and 13 (E13) exhibited genetic polymorphism.
目的在于探究2009年云南省与缅甸边境地区15岁以下健康儿童肠道病毒的监测情况。采集来自缅甸与云南省边境地区15岁以下健康儿童的粪便样本，对全部271份样本进行病毒分离及测序。从271份粪便中检测出6株脊髓灰质炎病毒（PVs），分离率为2.8%，均属于疫苗株，检测出24株非脊髓灰质炎病毒（NPVs），分离率为8.9%。24株NPVs属于B组人肠道病毒（HEV-B），有6个血清型，未分离出HEV-A、HEV-C和HEV-D病毒。其中，13株NPVs为E7（54.17%），5株NPVs为E13（20.83%）。结果显示，云南省边境地区肠道病毒携带率高于常规急性弛缓性麻痹（AFP）监测系统的报告率。分离出的肠道病毒仅为HEV-B病毒。系统发育分析表明，埃可病毒7型（E7）和13型（E13）呈现出基因多态性。
