Shibata Shingo, Suge Toshiyuki, Ishikawa Kunio, Matsuo Takashi
Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Am J Dent. 2011 Jun;24(3):148-52.
To evaluate the degree of penetration of an ammonium hexafluorosilicate [SiF: (NH4)2SiF6] solution containing various antibacterial agents into dentin and the depth of dentin tubule occlusion by the precipitate.
Various antibacterial SiF solutions were prepared with the addition to chlorhexidine (CHX), cetylpyridinium chloride (CPC), isopropyl methylphenol (IPMP), or epigallocatechin gallate (EGCG), respectively. Two types of dentin disks were prepared from extracted teeth. One was a dentin surface covered with a smear layer, and the other treated with EDTA for 2 minutes to remove the smear layer and open dentin tubules. Then, the disks were treated with SiF solution with or without antibacterial agents for 3 minutes. The dentin surface and a longitudinally divided surface were observed with scanning electron microscopy (SEM) immediately after SiF treatment and after immersion in synthetic saliva for 7 days.
SEM photographs demonstrated that dentin tubules after treatment with SiF were occluded homogeneously and similar to those on conventional SiF treatment regardless of the addition of an antibacterial agent. However, the depth of occlusion became significantly shallower when SiF was applied to dentin specimens covered with a smear layer.
评估含有各种抗菌剂的六氟硅酸铵[SiF：(NH4)2SiF6]溶液渗入牙本质的程度以及沉淀物对牙本质小管的封闭深度。
分别向洗必泰（CHX）、西吡氯铵（CPC）、异丙基甲基苯酚（IPMP）或表没食子儿茶素没食子酸酯（EGCG）中添加制备各种抗菌SiF溶液。从拔除的牙齿制备两种类型的牙本质盘。一种是覆盖有玷污层的牙本质表面，另一种用乙二胺四乙酸（EDTA）处理2分钟以去除玷污层并开放牙本质小管。然后，将牙本质盘用含或不含抗菌剂的SiF溶液处理3分钟。在SiF处理后以及浸泡在人工唾液中7天后，立即用扫描电子显微镜（SEM）观察牙本质表面和纵向剖开的表面。
SEM照片显示，无论是否添加抗菌剂，用SiF处理后的牙本质小管均被均匀封闭，与传统SiF处理后的情况相似。然而，当将SiF应用于覆盖有玷污层的牙本质标本时，封闭深度明显变浅。
