Food Futures Flagship, CSIRO Food and Nutritional Sciences, North Ryde, Australia.
Nutr Cancer. 2011;63(7):1074-84. doi: 10.1080/01635581.2011.596646. Epub 2011 Aug 29.
Kakadu plum (Terminalia ferdinandiana Exell, Combretaceae) and Illawarra plum (Podocarpus elatus Endl., Podocarpaceae) extracts were fractionated, using a bioassay-guided approach and screened for antioxidant activity [oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays] and antiinflammatory activity (nitrite concentration and prostaglandin E(2) release in lipopolysaccharide (LPS)-activated murine macrophages). Among 8 fractions obtained from KP and 5 fractions obtained from IP, fraction KPF5 from KP exhibited superior activity in all assays, with an ORAC value of 3,776 ± 603 μmol Trolox/g DW and a CAA value of 52.2 ± 8.6 μmol quercetin equivalents/g DW. In addition, KPF5 further demonstrated an upregulation of the Nrf2/Keap1 ratio in Hep G2 cells. KPF5 also inhibited the expression of COX-2 and iNOS in LPS-activated murine macrophages, potentially through the NF-κB, p44/42 mitogen activated protein kinase and Akt pathways. KPF5 also induced apoptosis and DNA damage in HT-29 cells, as determined by the cytokinesis block micronucleus cytome assay.
卡卡杜李（Terminalia ferdinandiana Exell，使君子科）和伊拉瓦拉李（Podocarpus elatus Endl.，罗汉松科）提取物经生物测定指导的方法进行分离，并针对抗氧化活性（氧自由基吸收能力（ORAC）和细胞抗氧化活性（CAA）测定）和抗炎活性（脂多糖（LPS）激活的巨噬细胞中的亚硝酸盐浓度和前列腺素 E（2）释放）进行筛选。从 KP 中得到的 8 个馏分和从 IP 中得到的 5 个馏分中，KP 的 KPF5 馏分在所有测定中表现出优越的活性，ORAC 值为 3,776 ± 603 μmol Trolox/g DW，CAA 值为 52.2 ± 8.6 μmol 槲皮素当量/g DW。此外，KPF5 还进一步在 Hep G2 细胞中上调了 Nrf2/Keap1 比值。KPF5 还通过 NF-κB、p44/42 丝裂原激活蛋白激酶和 Akt 途径抑制 LPS 激活的巨噬细胞中 COX-2 和 iNOS 的表达。通过细胞有丝分裂阻断微核细胞遗传学试验，KPF5 还诱导 HT-29 细胞凋亡和 DNA 损伤。
