5-[[(羧甲基)氨基]甲基]尿苷存在于酵母线粒体tRNA的反密码子中，这些tRNA识别以嘌呤结尾的双密码子家族。
5-[[(carboxymethyl)amino]methyl]uridine is found in the anticodon of yeast mitochondrial tRNAs recognizing two-codon families ending in a purine.
作者信息
Martin R P, Sibler A P, Gehrke C W, Kuo K, Edmonds C G, McCloskey J A, Dirheimer G
机构信息
Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
出版信息
Biochemistry. 1990 Jan 30;29(4):956-9. doi: 10.1021/bi00456a016.
The modified nucleoside (U*) present in the wobble position of Saccharomyces cerevisiae mitochondrial tRNA(Leu) and tRNA(Trp) was isolated by thin-layer chromatography and HPLC. Its chromatographic, UV spectral, and mass spectrometric properties were shown to be identical with those of 5- [[(carboxymethyl)amino]methyl]uridine (cmnm5U). This nucleoside found in yeast mitochondrial tRNAs reading two-codon families ending in a purine permits the selective recognition of A and G in the third codon position.
通过薄层色谱法和高效液相色谱法分离出了存在于酿酒酵母线粒体tRNA（Leu）和tRNA（Trp）摆动位置的修饰核苷（U*）。结果表明，其色谱、紫外光谱和质谱性质与5-[[(羧甲基)氨基]甲基]尿苷（cmnm5U）相同。在酵母线粒体tRNA中发现的这种核苷可识别以嘌呤结尾的双密码子家族，从而能够在第三个密码子位置选择性识别A和G。
Zotero 插件