真核生物的tRNA（脯氨酸）：反密码子环的一级结构；反密码子第一个核苷存在5-氨甲酰甲基尿苷或肌苷。
Eukaryotic tRNAs(Pro): primary structure of the anticodon loop; presence of 5-carbamoylmethyluridine or inosine as the first nucleoside of the anticodon.
作者信息
Keith G, Desgrès J, Pochart P, Heyman T, Kuo K C, Gehrke C W
机构信息
Institut de Biologie Moléculaire et Cellulaire du C.N.R.S. et de l'Université Louis Pasteur, Strasbourg, France.
出版信息
Biochim Biophys Acta. 1990 Jul 30;1049(3):255-60. doi: 10.1016/0167-4781(90)90095-j.
The modified nucleoside U*, located in the first position of the anticodon of yeast, chicken liver and bovine liver tRNA(Pro) (anticodon UGG), has been determined by means of TLC, HPLC, ultraviolet spectrum and gas chromatography-mass spectrometry. The structure was established as 5-carbamoylmethyluridine (ncm5U). In addition, we report on the primary structures of the above-mentioned tRNAs as well as those which have the IGG anticodon. In yeast, the two tRNA(Pro) (anticodons UGG and IGG) differ by eight nucleotides, whereas in chicken and in bovine liver, both anticodons are carried by the same 'body tRNA' with one posttranscriptional exception at position 32, where pseudouridine is associated with ncm5U (position 34) in tRNA(Pro) (U*GG) and 2'-O-methylpseudouridine is associated with inosine (position 34) in tRNA(Pro) (IGG).
位于酵母、鸡肝和牛肝tRNA(Pro)反密码子第一位的修饰核苷U*（反密码子为UGG），已通过薄层色谱法（TLC）、高效液相色谱法（HPLC）、紫外光谱法和气相色谱-质谱联用法得以确定。其结构确定为5-氨甲酰甲基尿苷（ncm5U）。此外，我们报道了上述tRNA以及具有IGG反密码子的tRNA的一级结构。在酵母中，两种tRNA(Pro)（反密码子分别为UGG和IGG）相差8个核苷酸，而在鸡肝和牛肝中，两种反密码子由同一个“主体tRNA”携带，仅在第32位有一个转录后差异，在tRNA(Pro)（U*GG）中，假尿苷与ncm5U（第34位）相关联，而在tRNA(Pro)（IGG）中，2'-O-甲基假尿苷与肌苷（第34位）相关联。
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