Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
Exp Parasitol. 2011 Nov;129(3):307-11. doi: 10.1016/j.exppara.2011.08.004. Epub 2011 Aug 22.
In order to investigate the factors determining the expulsion of intestinal helminths, we have analyzed the in vivo expression of IL-17, TGF-β and IL-23 in several tissues of two host species displaying different compatibility with Echinostoma caproni (Trematoda). We did not observe upregulation of these cytokines in any of the tissues of the high compatible host (mice). In contrast, the responses in the host of low compatibility (rats) with the parasite were markedly different. Significant increases in the expression of IL-17 and TGF-β were observed in the Peyer's patches and the intestine from the 2 to 8 weeks post-infection. The expression of IL-23 was upregulated from 2 to 4 weeks post-infection in the spleen, Peyer's patches and the intestine. Considering together our results with those published previously the development of chronic infections appears to be related with the development of local Th1 responses, whereas the early rejection of the worms is mediated by the development a biased Th17/Th2 phenotype. The Th17 response generated in rats may facilitate the worm expulsion via the suppression of the inflammatory Th1 responses and the increase in intestinal contractility.
为了研究决定肠道寄生虫排出的因素，我们分析了两种宿主物种体内几种组织中白细胞介素 17（IL-17）、转化生长因子-β（TGF-β）和白细胞介素 23（IL-23）的表达情况，这两种宿主物种对棘口吸虫（吸虫）的适应性不同。我们没有观察到高适应性宿主（小鼠）的任何组织中这些细胞因子的上调。相比之下，寄生虫低适应性宿主（大鼠）的反应明显不同。在感染后 2 至 8 周，派尔集合淋巴结和肠道中观察到 IL-17 和 TGF-β 的表达显著增加。在感染后 2 至 4 周，脾、派尔集合淋巴结和肠道中 IL-23 的表达上调。综合我们的结果和以前发表的结果，慢性感染的发展似乎与局部 Th1 反应的发展有关，而蠕虫的早期排斥则是由 Th17/Th2 表型的偏倚发展介导的。在大鼠中产生的 Th17 反应可能通过抑制炎症性 Th1 反应和增加肠道收缩性来促进蠕虫排出。
