State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, PR China.
Biomaterials. 2011 Dec;32(34):9031-9. doi: 10.1016/j.biomaterials.2011.08.032. Epub 2011 Aug 27.
The design and synthesis of multifunctional systems with high biocompatibility are very significant for the future of clinical applications. Herein, we report a microwave-assisted rapid synthesis of multifunctional Eu(3+)/Gd(3+) dual-doped hydroxyapatite (HAp) nanorods, and the photoluminescence (PL), drug delivery and in vivo imaging of as-prepared Eu(3+)/Gd(3+) doped HAp nanorods. The photoluminescent and magnetic multifunctions of HAp nanorods are realized by the dual-doping with Eu(3+) and Gd(3+). The PL intensity of doped HAp nanorods can be adjusted by varying Eu(3+) and Gd(3+) concentrations. The magnetization of doped HAp nanorods increases with the concentration of doped Gd(3+). The as-prepared Eu(3+)/Gd(3+)-doped HAp nanorods exhibit inappreciable toxicity to the cells in vitro. More importantly, the Eu(3+)/Gd(3+)-doped HAp nanorods show a high drug adsorption capacity and sustained drug release using ibuprofen as a model drug, and the drug release is governed by a diffusion process. Furthermore, the noninvasive visualization of nude mice with subcutaneous injection indicates that the Eu(3+)/Gd(3+)-doped HAp nanorods with the photoluminescent function are suitable for in vivo imaging. In vitro and in vivo imaging tests indicate that Eu(3+)/Gd(3+)-doped HAp nanorods have a potential in applications such as a multiple-model imaging agent for magnetic resonance (MR) imaging, photoluminescence imaging and computed tomography (CT) imaging. The Eu(3+)/Gd(3+) dual-doped HAp nanorods are promising for applications in the biomedical fields such as multifunctional drug delivery systems with imaging guidance.
多功能系统具有高生物相容性的设计和合成对于临床应用的未来非常重要。在此，我们报告了一种微波辅助快速合成多功能 Eu(3+)/Gd(3+) 双掺杂羟基磷灰石（HAp）纳米棒的方法，并研究了所制备的 Eu(3+)/Gd(3+) 掺杂 HAp 纳米棒的光致发光（PL）、药物输送和体内成像。通过 Eu(3+)和 Gd(3+) 的双掺杂，实现了 HAp 纳米棒的光致发光和磁性多功能。掺杂 HAp 纳米棒的 PL 强度可以通过改变 Eu(3+)和 Gd(3+)的浓度来调节。掺杂 HAp 纳米棒的磁化强度随掺杂 Gd(3+)浓度的增加而增加。所制备的 Eu(3+)/Gd(3+)-掺杂 HAp 纳米棒在体外对细胞几乎没有毒性。更重要的是，Eu(3+)/Gd(3+)-掺杂 HAp 纳米棒对布洛芬作为模型药物表现出高的药物吸附能力和持续的药物释放，并且药物释放由扩散过程控制。此外，对皮下注射的裸鼠进行的无创可视化表明，具有光致发光功能的 Eu(3+)/Gd(3+)-掺杂 HAp 纳米棒适用于体内成像。体外和体内成像测试表明，Eu(3+)/Gd(3+)-掺杂 HAp 纳米棒具有作为磁共振（MR）成像、光致发光成像和计算机断层扫描（CT）成像的多模式成像剂的潜在应用。Eu(3+)/Gd(3+) 双掺杂 HAp 纳米棒有望应用于多功能药物输送系统等生物医学领域，具有成像指导功能。
